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47 résultats pour "FINNZYMES REAGENTS"

"FINNZYMES REAGENTS"

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ADN polymérase Phusion® haute fidélité

ADN polymérase Phusion® haute fidélité

Supplier: FINNZYMES REAGENTS

L'ADN polymérase Phusion® génère des produits de la PCR avec une exactitude et une rapidité inaccessibles avec une seule enzyme, même sur les modèles les plus difficiles. La structure et les caractéristiques de l'ADN polymérase Phusion® haute fidélité en font le meilleur choix pour le clonage et redéfinissent les normes en matière de performance de PCR avec un taux d'erreurs 50 fois plus faible que pour l'ADN polymérase Taq et 6 fois plus faible que pour l'ADN polymérase Pfu. En outre, l'ADN polymérase Phusion® haute fidélité présente une processivité 10 fois supérieure à celle de l'ADN polymérase Pfu.

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Master mixes, multiplex, Phusion® U

Master mixes, multiplex, Phusion® U

Supplier: FINNZYMES REAGENTS

Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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Phusion® High-Fidelity PCR Master Mix with GC Buffer

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Master Mix with GC Buffer is a convenient 2X mix containing Phusion® DNA polymerase, nucleotides and optimised GC buffer including MgCl₂. Only template and primers need to be added by the user.

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Tissue direct PCR master mix, Phire™

Tissue direct PCR master mix, Phire™

Supplier: FINNZYMES REAGENTS

Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).

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DNA polymerases, Phusion® hot start II high-fidelity PCR master mix

DNA polymerases, Phusion® hot start II high-fidelity PCR master mix

Supplier: FINNZYMES REAGENTS

Phusion® high-fidelity DNA polymerases set a gold standard for high performance PCR.

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Phusion® Green Hot Start II High-Fidelity DNA Polymerase

Phusion® Green Hot Start II High-Fidelity DNA Polymerase

Supplier: FINNZYMES REAGENTS

Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

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ADN polymérase Phusion® Hot Start II haute fidélité

ADN polymérase Phusion® Hot Start II haute fidélité

Supplier: FINNZYMES REAGENTS

L'ADN polymérase Phusion® Hot Start II haute fidélité permet l'utilisation d'un large éventail d'amorces, y compris celles avec de faibles températures de fusion. L'ADN polymérase Phusion® Hot Start haute fidélité est idéal pour les applications de PCR, des PCR de routine aux applications très exigeantes, telles que le clonage et la PCR à haut rendement. Il est équipé d'un domaine à liaison d'ADN bicaténaire unique, ce qui rend cette ADN polymérase avec une activité de correction de type Pyrococcus extrêmement robuste, rapide et précise.

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DNA polymerases, Phusion® green U hot start

DNA polymerases, Phusion® green U hot start

Supplier: FINNZYMES REAGENTS

Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

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DyNAmo™ Flash SYBR® Green qPCR kit

DyNAmo™ Flash SYBR® Green qPCR kit

Supplier: FINNZYMES REAGENTS

DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

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Phusion® High-Fidelity PCR Master Mix

Phusion® High-Fidelity PCR Master Mix

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.

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DNA polymerases, Phusion® U hot start

DNA polymerases, Phusion® U hot start

Supplier: FINNZYMES REAGENTS

Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

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Phire® Green Hot Start II DNA Polymerase

Phire® Green Hot Start II DNA Polymerase

Supplier: FINNZYMES REAGENTS

Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

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Direct PCR Kits

Direct PCR Kits

Supplier: FINNZYMES REAGENTS

Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

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Phusion® site-directed mutagenesis kit

Phusion® site-directed mutagenesis kit

Supplier: FINNZYMES REAGENTS

Phusion® Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA.

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DyNAmo™ ColorFlash SYBR® Green qPCR kit

DyNAmo™ ColorFlash SYBR® Green qPCR kit

Supplier: FINNZYMES REAGENTS

DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

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Master mixes, hot start, Phusion® U

Supplier: FINNZYMES REAGENTS

Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

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