"Cytiva"

Supplier: Cytiva

Les colonnes HiTrap™ DEAE FF sont préremplies de DEAE Sepharose Fast Flow et constituent des échangeurs d'anions faibles pour les purifications de protéines à petite échelle ainsi que pour le criblage des conditions de liaison et d'élution.

Supplier: Cytiva

Idéal pour une purification performante des protéines taguées GST.

Supplier: Cytiva

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

Supplier: Cytiva

Les colonnes HiTrap™ CM FF dont préremplie de CM Sepharose™ Fast Flow et constituent des échangeurs de cations faibles pour les purifications de protéines à petite échelle ainsi que pour le criblage des conditions de liaison et d'élution.

Supplier: Cytiva

Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Supplier: Cytiva

Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.

Supplier: Cytiva

Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

Supplier: Cytiva

HiTrap™ Octyl FF are prepacked Octyl Sepharose™ 4 Fast Flow columns for hydrophobic interaction chromatography (HIC).

Supplier: Cytiva

HiScreen Octyl FF columns are prepacked with Octyl Sepharose 4 Fast Flow hydrophobic interaction chromatography (HIC) resins. The columns are an excellent choice for method optimization and parameter screening.

Supplier: Cytiva

HiScreen MabSelect is prepacked with MabSelect and is part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs).

Supplier: Cytiva

Joint torique, 13,1×1,6 mm, caoutchouc fluorocarboné (FPM)

Supplier: Cytiva

HiScreen™ Capto™ DEAE column is a weak anion exchanger with a bed height of 10 cm making it an excellent tool for method optimisation, parameter screening, robustness testing, and convenient scale-up.

Supplier: Cytiva

TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Supplier: Cytiva

CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.

Supplier: Cytiva

Amersham CyDye NIR-labeled secondary antibodies enable quantitation of Western blots with a strong signal-to-noise ratio. Mulitplexed experiments allow for the detection of two proteins on a single blot. The use of total lane signals to normalize target protein data yield robust and reliable results.

Supplier: Cytiva

Les marqueurs de blotting Amersham™ ECL™ DualVue™ remplissent deux fonctions principales. Tout d'abord, les marqueurs précolorés confirment le transfert des protéines, ainsi que l'orientation du transfert. Deuxièmement, les protéines recombinantes marquées sont détectées sur le film ou par imagerie CCD en conjonction avec la (les) protéine(s) cible(s) d'intérêt, permettant la détermination hautement précise de la masse moléculaire de la (des) protéine(s) cible(s). Les marqueurs sont détectés facilement et de façon spécifique par le biais d'un conjugué spécifique qui élimine les risques de contamination croisée entre la (les) protéine(s) cibles(s) et les marqueurs.