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Fluorometric polymerase activity assay kit, EvaEZ™

Supplier: Biotium

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

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PMA (Propidium monoazide) colorant se fixant à l'ADN/ARN

PMA (Propidium monoazide) colorant se fixant à l'ADN/ARN

Supplier: Biotium

PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.

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dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

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Grattoirs de cellules

Grattoirs de cellules

Supplier: Biotium

En PE, à usage unique, stérile. Mini grattoirs pour la récolte de cellules ou lysats cellulaires en microplaques à puits.

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qPCR master mixes, Forget-Me-Not™ EvaGreen®

qPCR master mixes, Forget-Me-Not™ EvaGreen®

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

    
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Wheat germ agglutinin (WGA) conjugates

Wheat germ agglutinin (WGA) conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

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Anti-KRT19 Mouse Monoclonal Antibody (CF488A) [clone: KRT19/799]

Supplier: Biotium

Recognizes a protein of 40 kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

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One-Step Blue® protein gel stain

One-Step Blue® protein gel stain

Supplier: Biotium

One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.

    
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Hydroxystilbamidine bis(methanesulphonate) 4% dans l'eau colorant fluorescent, pour histologie

Hydroxystilbamidine bis(methanesulphonate) 4% dans l'eau colorant fluorescent, pour histologie

Supplier: Biotium

Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. The 4% solution in deionized H2O is a convenient, ready-to-use form..

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Colorations d'acides nucléiques, PAGE GelRed™

Colorations d'acides nucléiques, PAGE GelRed™

Supplier: Biotium

Colorants conçus pour l'ADN sur gels de polyacrylamide.

    
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Cell viability assay kits

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

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Pluronic® F-127 20% dans le DMSO

Supplier: Biotium

Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as cell-permeable AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 calcium indicators to facilitate cell loading.

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Paraformaldéhyde 4% dans un tampon phosphate salin prêt-à-l'emploi, réactif fixateur

Supplier: Biotium

Paraformaldehyde, 4% in PBS is a ready-to-use fixation solution for cells or tissues.

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Diméthylsulfoxyde, anhydre ≥99.9%

Supplier: Biotium

Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions.

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PMA-Lite™ 2.0 LED Photolysis Device

Supplier: Biotium

The PMA-Lite™ 2.0 LED photolysis device is a light-weight LED light box specifically designed for optimal photoactivation of samples treated with propidium monoazide (PMA), PMAxx™ or ethidium monoazide (EMA) in viability PCR applications.

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