- Durée du test:Multiple steps
- Type de test:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Cible:UCP3
- Désignation:Human UCP3 ELISA kit
- Type déchantillon:Serum, plasma, tissue homogenates, and other biological fluids
- Réactivité croisée:Human UCP3 ELISA Kit exhibits high specificity and excellent specificity for the detection of human UCP3. No significant cross-reactivity or interference between UCP3 and analogues was observed.
- Méthode de détection:Colorimetric
- Time to Results:4 hours
- Durée de conservation:Store for 6 months at 4 °C
- Portée de détection:31,25 - 2000 pg/ml
- Température de stockage:4 °C
- Volume d'échantillon:100 μl
- Sensibilité:18,75 pg/ml
- Regulatory status:RUO
- Tests par set:96 tests
Human UCP3 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human UCP3 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human UCP3 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human UCP3 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for UCP3 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the UCP3 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-UCP3 Antibody, which binds the captured UCP3 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of UCP3 captured in each well. The concentration of UCP3 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.