Specifications
- Durée du test:Multiple steps
- Type de test:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Cible:PSMD8
- Désignation:Human PSMD8 ELISA kit
- Type déchantillon:Serum, plasma, tissue homogenates, and other biological fluids
- Réactivité croisée:Human PSMD8 ELISA Kit exhibits high specificity and excellent specificity for the detection of human PSMD8. No significant cross-reactivity or interference between PSMD8 and analogues was observed.
- Méthode de détection:Colorimetric
- Time to Results:4 hours
- Durée de conservation:Store for 6 months at 4 °C
- Portée de détection:0,156 - 10 ng/ml
- Température de stockage:4 °C
- Volume d'échantillon:100 μl
- Sensibilité:0,094 ng/ml
- Regulatory status:RUO
- Tests par set:96 tests
Specifications
About this item
Human PSMD8 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human PSMD8 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human PSMD8 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human PSMD8 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for PSMD8 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PSMD8 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PSMD8 Antibody, which binds the captured PSMD8 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PSMD8 captured in each well. The concentration of PSMD8 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.