The Amicon® Pro device streamlines the affinity purification process by providing a single adaptable centrifugation unit capable of performing all steps in the affinity purification process. The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange, eliminating the need for multiple sample transfers, thereby minimising protein loss.

• Single device
• Fewer spins
• Greater sample recovery
• Volume: 10 ml
• Minimum final concentrate volume: 15 L
• Color code: Clear with pink cap

The large reservoir reduces the need for multiple centrifugation steps. Attaching the Amicon® 0,5 ml filter during the elution step provides simultaneous concentration and the option of highly efficient diafiltration (>99%) in a single spin.

These centrifugal filter units can be used for a number of applications: 3 kDa Centrifugal tool collapsing protein purification protein concentration, buffer exchange, protein enrichment and antibody labeling.

The Amicon® Pro Purification system eliminates the need for multiple sample transfers in the purification workflow thereby minimising protein loss. Direct coupling to an Amicon Ultra-0,5 device provides simultaneous concentration during the elution phase, a feature particularly advantageous for depletion or enrichment applications.The Amicon® Pro purification system offers highly efficient diafiltration, permitting buffer exchange and concentration of the final purified sample in a single 15-minute spin.

Device Material
Collection tube, holder tube, exchange device and AU0,5 collection tube: Polypropylene
Cap: Polyethylene
Frit: Polypropylene/polyethylene
Amicon Ultra Device: Styrene/butadiene

Suitable techniques are buffer exchange, protein purification, protein purification and protein purification.