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EDTA Buffer, pH 8,5, 10X, Antigen Retriever
EDTA Buffer, pH 8,5, 10X, Antigen Retriever
Référence E1161-1000ML
Fournisseur:  SIGMA ALDRICH MICROSCOPY
Numéro CAS:  
EDTA Buffer, pH 8,5, 10X, Antigen Retriever
Référence E1161-1000ML
Fournisseur:  SIGMA ALDRICH MICROSCOPY
Numéro CAS:  

Spécifications

  • Cdt:
    1.000 mL
  • Désignation:
    EDTA buffer, 10X, antigen retriever
  • Température de stockage:
    2...8 °C
  • Physical form:
    Liquid
  • Valeur pH:
    8,5

Spécifications

À propos de cet article

Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of EDTA buffer, pH 8,5, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens. EDTA buffer has been used to heat tissue sections in order to visualise antigens by immunohistochemical analysis.