This antibody recognises multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.

Flow Cytometry: use the Multi Cytokeratin mAb at 0,5-1 µg/million cells IF: 0,5 - 1 µg/ml WB: 0,5 - 1 µg/ml IHC (FFPE): 0,5 - 1 µg/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6,0, for 10 - 20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]
Clonality: Monoclonal
Clone: C11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Bovine, Rat, Mouse, Guinea pig, Frog, Goat, Marmoset, Pig