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RPMI 1640 Medium Modified without L-Glutamine, without Amino acids, Glucose, Phenol Red (Powder)
RPMI 1640 Medium Modified without L-Glutamine, without Amino acids, Glucose, Phenol Red (Powder)
Référence USBIR9010-02-10L
Fournisseur:  US Biological
Numéro CAS:  
RPMI 1640 Medium Modified without L-Glutamine, without Amino acids, Glucose, Phenol Red (Powder)
Référence USBIR9010-02-10L
Fournisseur:  US Biological
Numéro CAS:  

Spécifications

  • Format du milieu:
    Powder
  • Cdt:
    1
  • Désignation:
    RPMI 1640 medium modified without L-glutamine, without amino acids, glucose, phenol red (powder)
  • Taille:
    10 L
  • Phenol red:
    No
  • L-Glutamine:
    No
  • Niveau de glucose:
    Without
  • Media Base:
    RPMI 1640 - Milieu Roswell Park Memorial Institute Medium
  • Température de stockage:
    Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 °C and used within a short period of time.
  • Température de transport:
    Powder, RT

Spécifications

À propos de cet article

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI.

  • Appearance: White to light yellow homogenous, free flowing powder
  • Solubility: Colourless, clear, complete
  • Endotoxin: ≤1 EU/ml

Directions per Liter: Dissolve 7,4 grams in 900 ml of ddH₂O stirring gently until completely solubilised. Do NOT heat. If required, add 2 g sodium bicarbonate with stirring. Adjust pH of the medium to 0,1 to 0,3 pH unit below the desired pH. Add additional water to bring the solution to 1 L. Filter-sterilise using a 0,22 µm membrane. Aliquot into sterile containers.