When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.

• Pour 6 groupes de laboratoire
• Empaquetage et analyse des colonnes : 45 minutes ; électrophorèse facultative : 60 minutes ; coloration : 30 minutes ; décoloration : 2 heures

Autres éléments nécessaires : bain-marie, lampe UV à ondes longues, support annulaire et pinces, micropipette automatique, système d'électrophorèse verticale sur gel, alimentation, gels de polyacrylamide (12%).