A competitive assay for the quantification of corticosterone in serum and rodent feces.

• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure corticosterone levels down to 30 pg/ml
• Incubation: Overnight | Development: 90 – 120 minutes | Read: Colorimetric at 405 – 420 nm
• Mid-point: 50% B/B₀: 130 – 180 pg/ml

Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (adrenocorticotropic hormone), and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress. Plasma corticosterone levels have a circadian variation, and may be important in the regulation of the sleep-wake cycle. Cayman’s Corticosterone ELISA Kit is a competitive assay that can be used for quantification of corticosterone in serum and rodent feces.