Taq ADN polymérase
À propos de cet article
VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.
- Ideal choice for routine applications
- High performance, thermostable DNA polymerase
- Optimal for TA cloning
Taq DNA polymerase concentration: 5 Units/μl
10X Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8,3; KCl, 15 mM MgCl₂, 1% Triton™ X-100
10X Mg-Free Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 1% Tween® 20
10X Mg-Free Extra Buffer: Tris-HCl pH 8,3; KCl, 1% Triton™ X-100
EU = Units
: VWR® Taq DNA Polymerase is usually supplied with either or both Key Buffer and Extra Buffer. Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) minimising the need for optimisation of the Mg²⁺ concentration, or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K⁺) buffer. Extra Buffer promotes high specificity, but careful optimisation of primer annealing temperatures and Mg²⁺ concentrations may be required.
12 variantes
Product Details & Documents
VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.
- Ideal choice for routine applications
- High performance, thermostable DNA polymerase
- Optimal for TA cloning
Taq DNA polymerase concentration: 5 Units/μl
10X Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8,3; KCl, 15 mM MgCl₂, 1% Triton™ X-100
10X Mg-Free Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 1% Tween® 20
10X Mg-Free Extra Buffer: Tris-HCl pH 8,3; KCl, 1% Triton™ X-100
EU = Units
: VWR® Taq DNA Polymerase is usually supplied with either or both Key Buffer and Extra Buffer. Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) minimising the need for optimisation of the Mg²⁺ concentration, or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K⁺) buffer. Extra Buffer promotes high specificity, but careful optimisation of primer annealing temperatures and Mg²⁺ concentrations may be required.
Certificats
Rechercher par numéro de lotSDS_FR_CH_VWRC733-1300 SDS_FR_CH_VWRC733-1301 SDS_FR_CH_VWRC733-1302 SDS_FR_CH_VWRC733-1303 SDS_FR_CH_VWRC733-1312
TDS Taq DNA Polymerase, VWR TDS VWR Taq DNA Polymerase 1000 Units TDS VWR Taq DNA Polymerase 1000 Units with 10x Extra Buffer (Mg2+ free and Triton free) TDS VWR Taq DNA Polymerase 1000 Units with 10x Key Buffer (15 mM MgCl2) with 10x Extra Buffer (15 mM MgCl2) TDS VWR Taq DNA Polymerase 1000 Units with 10x Key Buffer (15 mM MgCl2, Tween free)
Spécifications de la famille de produits
Spécifications
Spécifications
- Catalog No:
- 733-1300
- 733-1301
- 733-1302
- 733-1819
- 733-1820
- 733-1303
- 733-1312
- 733-1313
- 733-2009
- 733-1305
- 733-1307
- 733-1823
- Désignation:
- Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂
- Taq DNA Polymerase, tampon Extra sans MgCl₂ 10X, 25 mmol de MgCl₂
- Taq DNA Polymerase, tampon Key sans Tween 10X (15 mM MgCl₂), tampon Extra sans Triton 10X (15 mM MgCl₂), 25 mmol de MgCl₂
- Taq DNA Polymerase, tampon Key sans Tween 10X (15 mM MgCl₂), tampon Extra sans Triton 10X (15 mM MgCl₂), 25 mmol de MgCl₂
- Format:
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Enzyme with/without buffer
- Caractéristiques:
- Mg-free
- Mg-free
- Mg-free
- Detergent free
- Detergent free
- Detergent free
- Démarrage à chaud:
- No
- No
- No
- No
- No
- No
- No
- No
- No
- No
- No
- No
- Concentration:
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- 5 U/µl
- Systèmes:
- 250
- 500
- 1000
- 2500
- 5000
- 10000
- 1000
- 2500
- 10000
- 1000
- 1000
- 10000
- Taille:
- 1 x 0.05 ml + 3 x 1.5 ml
- 1 x 0.1 ml + 3 x 1.5 ml
- 2 x 0.1 ml + 6 x 1.5 ml
- 5 x 0.1 ml + 15 x 1.5 ml
- 10 x 0.1 ml + 9 x 5 ml
- 3 x 0.667 ml + 18 x 5 ml
- 2 x 0.1 ml + 4 x 1.5 ml
- 5 x 0.1 ml + 10 x 1.5 ml
- 3 x 0.667 ml + 12 x 5 ml
- 2 x 0.1 ml + 4 x 1.5 ml
- 2 x 0.1 ml + 4 x 1.5 ml
- 3 x 0.667 ml + 18 x 5 ml
- Composition du tampon:
- 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
- 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
- 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
- 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
- 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
- 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
- 10x Key Buffer Mg-free and 25 mM MgCl2
- 10x Key Buffer Mg-free and 25 mM MgCl2
- 10x Key Buffer Mg-free and 25 mM MgCl2
- 10x Extra Buffer and 25 mM MgCl2
Detergent free - 10x Key Buffer and 25 mM MgCl2
Detergent free - 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Detergent free
- Haute fidélité:
- Non
- Non
- Non
- Non
- Non
- Non
- Non
- Non
- Non
- Non
- Non
- Non
- Taille de l'amplicon:
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- ≤ 5kb
- Processivity:
- Standard
- Standard
- Standard
- Standard
- Standard
- Standard
- Standard
- Standard
- Standard
- Standard
- Standard
- Standard
- Activité exonucléase:
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- 5’→3’
- Product overhang:
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- 3'A
- Conditions de stockage:
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- – 20 °C (long term), + 4 °C (6 months)
- Application:
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
- Standard amplification
Spécifications
Product Family Options
Informations sur le produit
- DésignationCdtDisponibilitéPrix
- Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂250 EUConnexion Pour la disponibilité1 * 250 UESFr. 90.50Spécifications :
Plus
Specifications
Cat. No.733-1300DésignationTaq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDémarrage à chaudNoConcentration5 U/µlSystèmes250Taille1 x 0.05 ml + 3 x 1.5 mlComposition du tampon10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂500 EUConnexion Pour la disponibilité1 * 500 UESFr. 149.00Spécifications :
Plus
Specifications
Cat. No.733-1301DésignationTaq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDémarrage à chaudNoConcentration5 U/µlSystèmes500Taille1 x 0.1 ml + 3 x 1.5 mlComposition du tampon10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂1.000 EUConnexion Pour la disponibilité1 * 1.000 UESFr. 317.00Spécifications :
Plus
Specifications
Cat. No.733-1302DésignationTaq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDémarrage à chaudNoConcentration5 U/µlSystèmes1000Taille2 x 0.1 ml + 6 x 1.5 mlComposition du tampon10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂2.500 EUConnexion Pour la disponibilité1 * 2.500 UESFr. 707.00Spécifications :
Plus
Specifications
Cat. No.733-1819DésignationTaq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDémarrage à chaudNoConcentration5 U/µlSystèmes2500Taille5 x 0.1 ml + 15 x 1.5 mlComposition du tampon10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂5.000 EUConnexion Pour la disponibilité1 * 5.000 UESFr. 1’290.00Spécifications :
Plus
Specifications
Cat. No.733-1820DésignationTaq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDémarrage à chaudNoConcentration5 U/µlSystèmes5000Taille10 x 0.1 ml + 9 x 5 mlComposition du tampon10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂10.000 EUConnexion Pour la disponibilité1 * 10.000 UESFr. 1’950.00Spécifications :
Plus
Specifications
Cat. No.733-1303DésignationTaq DNA Polymerase, tampon Key 10X (15 mM MgCl₂), tampon Extra 10X (15 mM MgCl₂), 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDémarrage à chaudNoConcentration5 U/µlSystèmes10000Taille3 x 0.667 ml + 18 x 5 mlComposition du tampon10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂1.000 EUConnexion Pour la disponibilité1 * 1.000 UESFr. 310.00Spécifications :
Plus
Specifications
Cat. No.733-1312DésignationTaq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesMg-freeDémarrage à chaudNoConcentration5 U/µlSystèmes1000Taille2 x 0.1 ml + 4 x 1.5 mlComposition du tampon10x Key Buffer Mg-free and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂2.500 EUConnexion Pour la disponibilité1 * 2.500 UESFr. 688.00Spécifications :
Plus
Specifications
Cat. No.733-1313DésignationTaq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesMg-freeDémarrage à chaudNoConcentration5 U/µlSystèmes2500Taille5 x 0.1 ml + 10 x 1.5 mlComposition du tampon10x Key Buffer Mg-free and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂10.000 EUConnexion Pour la disponibilité1 * 10.000 UESFr. 1’940.00Spécifications :
Plus
Specifications
Cat. No.733-2009DésignationTaq DNA Polymerase, tampon Key sans MgCl₂ 10X, 25 mM de MgCl₂FormatEnzyme with/without bufferCaractéristiquesMg-freeDémarrage à chaudNoConcentration5 U/µlSystèmes10000Taille3 x 0.667 ml + 12 x 5 mlComposition du tampon10x Key Buffer Mg-free and 25 mM MgCl2Haute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Extra sans MgCl₂ 10X, 25 mmol de MgCl₂1.000 EUConnexion Pour la disponibilité1 * 1.000 UESFr. 310.00Spécifications :
Plus
Specifications
Cat. No.733-1305DésignationTaq DNA Polymerase, tampon Extra sans MgCl₂ 10X, 25 mmol de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDetergent freeDémarrage à chaudNoConcentration5 U/µlSystèmes1000Taille2 x 0.1 ml + 4 x 1.5 mlComposition du tampon10x Extra Buffer and 25 mM MgCl2
Detergent freeHaute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key sans Tween 10X (15 mM MgCl₂), tampon Extra sans Triton 10X (15 mM MgCl₂), 25 mmol de MgCl₂1.000 EUConnexion Pour la disponibilité1 * 1.000 UESFr. 317.00Spécifications :
Plus
Specifications
Cat. No.733-1307DésignationTaq DNA Polymerase, tampon Key sans Tween 10X (15 mM MgCl₂), tampon Extra sans Triton 10X (15 mM MgCl₂), 25 mmol de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDetergent freeDémarrage à chaudNoConcentration5 U/µlSystèmes1000Taille2 x 0.1 ml + 4 x 1.5 mlComposition du tampon10x Key Buffer and 25 mM MgCl2
Detergent freeHaute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification - Taq DNA Polymerase, tampon Key sans Tween 10X (15 mM MgCl₂), tampon Extra sans Triton 10X (15 mM MgCl₂), 25 mmol de MgCl₂10.000 EUConnexion Pour la disponibilité1 * 10.000 UESFr. 1’950.00Spécifications :
Plus
Specifications
Cat. No.733-1823DésignationTaq DNA Polymerase, tampon Key sans Tween 10X (15 mM MgCl₂), tampon Extra sans Triton 10X (15 mM MgCl₂), 25 mmol de MgCl₂FormatEnzyme with/without bufferCaractéristiquesDetergent freeDémarrage à chaudNoConcentration5 U/µlSystèmes10000Taille3 x 0.667 ml + 18 x 5 mlComposition du tampon10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Detergent freeHaute fidélitéNonTaille de l'amplicon≤ 5kbProcessivityStandardActivité exonucléase5’→3’Product overhang3'AConditions de stockage– 20 °C (long term), + 4 °C (6 months)ApplicationStandard amplification



