Specifications

• Conjugaison:
  Unconjugated
• Type de protéine/peptide:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His Tag (SPR & BLI verified)
• Conditions de stockage:
  –20 °C
• Teneur en endotoxines:
  Less than 1,0 EU per μg by the LAL method.
• Activité biologique:
  Immobilised Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 10,1 μM as determined in a SPR assay (Biacore T200) (QC tested). ; Immobilised Rituximab on CM5 Chip, can bind Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) with an affinity constant of 5,5 μM as determined in a SPR assay (Biacore T200) (Routinely tested). ; Loaded Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 4,30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Rituximab on FAB2G Biosensor, can bind Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) with an affinity constant of 5,4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• ID de gène:
  P31994
• Synonyme de protéine:
  CD32|IGFR2|CD32b|FCG2|CDw32|FCGR2B
• Nom de la protéine/du peptide:
  Fc gamma RIIB/ CD32b
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular weight:
  20,4 kDa
• Niveau d'endotoxine:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Température de transport:
  RT