Human recombinant IL-7 R alpha (from HEK293)
- Conjugaison:Unconjugated
- Type de protéine/peptide:Recombinant
- Source:HEK293 cells
- Species:Human
- Conditions de stockage:–20 °C
- Teneur en endotoxines:Less than 1,0 EU per μg by the LAL method.
- Activité biologique:Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised ActiveMax® Human IL-7, Tag Free (Cat. No. ACRBIL7-H4219) at 2 μg/ml (100 μl/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. ACRBIL7-H5258) with a linear range of 4-31 ng/ml (QC tested).; Measured by its binding ability in a functional ELISA. Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. ACRBTSP-H82E0) immobilised at 2 μg/ml (100 μl/well) via precoated 5 μg/ml (100 μl/well) of Human TSLP R, Fc Tag (Cat. No. ACRBTSR-H525a), can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. ACRBIL7-H5258) with a linear range of 1 - 20 ng/ml (Routinely tested).; Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. ACRBIL7-H5258) on CM5 chip via anti-mouse antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. ACRBIL7-H4219) with an affinity constant of 13,3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
- ID de gène:P16871-1
- Sans vecteur:Y
- Exempt de protéase:Y
- Exempt de substance animale:Y
- Synonyme de protéine:IL7Ra|CD127
- Nom de la protéine/du peptide:IL-7 R alpha
- Purity:>90%
- Molecular weight:52,0 kDa
- Niveau d'endotoxine:Low
- Formulation:Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
- Absence de nucléases:Y
- Température de transport:Powder,RT
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