Human recombinant Glypican 3 (from HEK293), Acro Biosystems
- Conjugaison:Unconjugated
- Type de protéine/peptide:Recombinant
- Source:HEK293 cells
- Species:Human
- Conditions de stockage:–20 °C
- Teneur en endotoxines:Less than 0,1 EU per μg by the LAL method.
- Activité biologique:Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised Human FGF basic, Tag Free (Cat. No. ACRBBFF-H4117) at 0.5 μg/ml (100 μl/well) can bind Human Glypican 3, His Tag (Cat. No. ACRBGP3-H52H4) with a linear range of 8 - 31 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised Human Glypican 3, His Tag (Cat. No. ACRBGP3-H52H4) at 0,5 μg/ml (100 μl/well) can bind Anti-Human GPC3 MAb with a linear range of 0,1 - 2 ng/ml (Routinely tested). Anti-human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3, His Tag (Cat. No. ACRBGP3-H52H4) with an affinity constant of 1,33 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3, His Tag (Cat. No. ACRBGP3-H52H4) with an affinity constant of 1,48 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- ID de gène:P51654-1
- Sans vecteur:Y
- Exempt de protéase:Y
- Exempt de substance animale:Y
- Synonyme de protéine:GPC3|SGBS|SGB|Glypican-3|SDYS |MXR7|SGBS1|OCI5|GTR2-2|DGSX
- Nom de la protéine/du peptide:Glypican 3
- Purity:>90%
- Molecular weight:38,1 kDa and 24,6 kDa
- Niveau d'endotoxine:Low
- Formulation:Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
- Absence de nucléases:Y
- Température de transport:Powder,RT
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