Spécifications

• Conjugaison:
  Unconjugated
• Type de protéine/peptide:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Conditions de stockage:
  –20 °C
• Teneur en endotoxines:
  Less than 0,1 EU per μg by the LAL method.
• Activité biologique:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4, Normally trehalose is added as protectant before Lyophilisation,; Measured by its binding ability in a functional ELISA, Immobilised Human PD-L1, Fc Tag (HPLC-verified) (Cat, No, PD1-H5258) at 10 μg/ml (100 μl/well) can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat, No, PD1-H5255) with a linear range of 2-40 ng/ml (QC tested),; Measured by its binding ability in a functional ELISA, Immobilised Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat, No, PD1-H5255) at 2 μg/ml (100 μl/well) can bind Human PD-L2, Fc Tag (Cat, No, PD2-H5251) with a linear range of 0,2-4 ng/ml (Routinely tested),; Measured by its binding ability in a functional ELISA, Immobilised Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat, No, PD1-H5255) at 2 μg/ml (100 μl/well) can bind Nivolumab with a linear range of 0,1-2 ng/ml (Routinely tested),; Measured by its binding ability in a functional ELISA, Immobilised Human PD-L1, His Tag (HPLC-verified) (Cat, No, PD1-H5229) at series of concentration on MonRabTM Anti-His Tag (C-term) Antibody precoated (0,1 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat, No, PD1-H5255) at 5 μg/ml (100 μl/well) with a linear range of 10-78 ng/ml (Routinely tested),; Loaded Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat, No, PD1-H5255) on Protein A Biosensor, can bind Human PD-L1, His Tag (HPLC verified) (Cat, No, PD1-H5229) with an affinity constant of 2,6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested),; Loaded Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat, No, PD1-H5255) on Protein A Biosensor, can bind Human PD-L2, His Tag (SPR verified) (Cat, No, PD2-H5220) with an affinity constant of 0,43 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• ID de gène:
  Q15116-1
• Sans vecteur:
  Y
• Exempt de protéase:
  Y
• Exempt de substance animale:
  Y
• Synonyme de protéine:
  CD279|PDCD1|PD1|SLEB2
• Nom de la protéine/du peptide:
  PD-1
• Purity:
  >95%
• Molecular weight:
  42,5 kDa
• Niveau d'endotoxine:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4, Normally trehalose is added as protectant before Lyophilisation.
• Absence de nucléases:
  Y
• Température de transport:
  Powder,RT