SURE competent cells
À propos de cet article
SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.
- Lack components of the pathways that catalyse the rearrangement and deletion of nonstandard secondary and tertiary structures
- Allows for blue-white colour screening
Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems.
SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryotic cells. SURE cells are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-) endonuclease (endA) deficient, and recombination (recB recJ) deficient.
: For research use only. Not for use in diagnostic procedures.
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Product Details & Documents
SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.
- Lack components of the pathways that catalyse the rearrangement and deletion of nonstandard secondary and tertiary structures
- Allows for blue-white colour screening
Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems.
SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryotic cells. SURE cells are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-) endonuclease (endA) deficient, and recombination (recB recJ) deficient.
: For research use only. Not for use in diagnostic procedures.
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Informations sur le produit
- DésignationCaractéristiqueInfo cdtDisponibilitéPrix
- SURE electroporation-competent cellsFor cloning DNA with secondary structures, ≥1×10¹⁰ transformants/µg5×0,1 mlConnexion Pour la disponibilité1 * 5 STSFr. 454.00
- SURE competent cellsFor routine cloning of DNA with secondary structures, ≥5×10⁸ transformants/µg5×0,2 mlConnexion Pour la disponibilité1 * 1 mLSFr. 433.00
- SURE 2 supercompetent cellsHigh-efficiency derivative for cloning DNA with secondary structures, >1×10⁹ transformants/µg10×0,1 mlConnexion Pour la disponibilité1 * 1 mLSFr. 442.00
#: 20864.320
Fournisseur: VWR Chemicals
#: 83640.320
Fournisseur: VWR Chemicals
#: 1.00030.2500
Fournisseur: Merck
#: 31770.294
Fournisseur: VWR Chemicals
#: 83645.320
Fournisseur: VWR Chemicals
#: 1.09439.1000
Fournisseur: Merck