Fluor-de-Lys® Developer I concentrate
Product Details & Documents
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
- An essential reagent for signal generation in HDAC or sirtuin assays performed with the FLUOR DE LYS® substrate.
Specifications
Specifications
- Cdt:300 µl
- Désignation:Fluor-de-Lys® Developer I concentrate
- Cat. No.:ENZOBMLKI1050300
- Concentration:20X concentrate. After dilution (1X), sufficient for 100 assays (50 µl reaction volume)
Specifications
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