Specifications
- Cdt:50 µG
- Type de protéine/peptide:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:His Tag
- Conditions de stockage:Working aliquots are stable for up to 3 months when stored at −20 °C.
- Activité biologique:>2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol of NAD+ per min.
- Synonyme de protéine:Nicotinamide Mononucleotide Adenylyltransferase 3|NMN Adenylyltransferase 3|NaMN Adenylyltransferase 3|EC 2.7.7.1|EC 2.7.7.18
- Nom de la protéine/du peptide:NMNAT3 (highly active)
- Purity:>95% (SDS-PAGE)
- Sequence:1-252
- Formulation:Liquid, In 50mM TRIS-HCl, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
Specifications
About this item
Mitochondrial NMNAT isoform. Catalyses the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyses the reverse reaction, i. e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury.
Fusion-Tag: His Tag
This recombinant proteins is for research use only.