Recombinant

E. coli

Human

Lyophilized Retinol binding protein-4 although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution Retinol binding protein-4 should be stored at 4 °C between 2-7 days and for future use below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please prevent freeze/thaw cycles.

Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5M retinol acids/per RBP4.

Retinol Binding

RBP4 His

Greater than 95.0% as determined by RP-HPLC and analysis by SDS-PAGE

It is recommended to reconstitute the lyophilized Retinol binding protein-4 in sterile deionized water to working volume of 0.5mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate st

MSWWHHHHHH NWNIPTTQDT TERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKG RVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSERNLL.

RBP-4 His tag was filtered (0.4 um) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5.

SDS-PAGE