The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.

• Optimised trypsin digestion producing complete digests of protein ranging from 0,025 to 10 µg
• Optimised guanidination where reaction produces little or no N-terminal modification
• Protein can be digested and guanidinated all in one day

Accurate identification of proteins and analysis of post-translational modifications by mass spectrometry require accurate and complete protein digestion and peptide modification and this kit provides an optimised procedure and reagents for approximately 90 digests.