Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.

• For affinity chromatography
• p-aminobenzamidine (pABA) immobilized on Sepharose 4 Fast Flow matrix gives high productivity, and allows easy scale-up
• Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins
• Amide coupling method
• Stable in all commonly used aqueous buffers

Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates. Benzamidine Sepharose™ 4 Fast Flow (High Sub) is based on highly cross-linked 4% agarose enabling high flow rates without losing binding capacity.

For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.