MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.

• High-flow agarose matrix results in high flow velocities at production scale allowing for the processing of more than 10000 litres of feed in one working day
• Oriented coupling of the ligand and optimised matrix gives high dynamic binding capacity which reduces resin volume requirements
• Low non specific binding resulting in low levels of impurities in the product eluate pool
• BioProcess resin supported for industrial applications and well-established in approved processes

MabSelect™ has been developed for cost-effective high throughput processing of large amounts of feed. This is the reason why MabSelect™ has been designed-in to several new and second-generation processes for the production of therapeutic mAbs. The resin is based on a highly cross-linked agarose matrix with a recombinant protein A ligand. This ligand is engineered to favour an oriented coupling resulting in an affinity resin with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. MabSelect™ is optimised for high capacity, throughput and to deliver a product pool that is high in purity and yield.

The recombinant protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect an excellent choice for purification of mAbs at process scale.