Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

• Cost-efficient gel filtration media providing high resolution with fast and easy operation
• Five different fractionation ranges available in Sephacryl HR family allows for reproducible separations over a wide molecular weight range
• BioProcess medium supported for industrial applications and well-established in approved processes

Sephacryl™ High Resolution chromatography media is a highly versatile gel filtration media that offers a wide range of fractionation capabilities. The matrix of Sephacryl™ High Resolution media is a cross-linked copolymer of allyl dextran and N,N’-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the media minimises non specific adsorption and maximises recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl™ HR the medium of choice for routine purification.

Sephacryl™ High Resolution is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As a BioProcess medium, Sephacryl High Resolution, meets industrial demands with security of supply and comprehensive technical and regulatory support.

Sephacryl™ S-100 High Resolution: For separating peptides and small proteins (fractionation range globular proteins 1×10³ to 1×10⁵).
Sephacryl™ S-200 High Resolution: For purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5×10³ to 2.5×10⁵).
Sephacryl™ S-300 High Resolution: For purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1×10⁴ to 1.5×10⁶).
Sephacryl™ S-400 High Resolution: To separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2×10⁴ to 8×10⁶).
Sephacryl™ S-500 High Resolution: To separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4×10⁴ to 2×10⁷)