Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Ethidium bromide adsorber for decontamination of ethidium bromide from staining solutions, Sigma-Aldrich®
Supplier: Merck
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.
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Sachets de décoloration
Supplier: VWR Chemicals
Les sachets de décoloration sont conçus pour éliminer en toute sécurité le bromure d'éthidium des solutions pendant un traitement d'une nuit. Chaque sachet peut extraire jusqu'à 5 mg de bromure d'éthidium de la solution, ce qui permet de minimiser l'exposition du personnel de recherche à ce matériau toxique. Le mélange absorbant spécial permet retenir les molécules du colorant dans un sachet pratique pour une élimination par incinération.
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Nucleic acid and protein destain, Silver D-Stain™
Supplier: G-Biosciences
The staining of gels with silver stains often results in over-staining or unevenly stained gels. Silver D-Stain™ eliminates the need to repeat the electrophoresis.
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Decontamination bags, activated charcoal
Supplier: Biotium
Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.
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Destain solutions
Supplier: G-Biosciences
G-Biosciences' Destain I contains approximately 45% methanol and 9% glacial acetic acid. G-Biosciences' Destain II contains approximately 5% methanol and 7% acetic acid.
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Ethidium bromide Greenbag™ disposal kit, MP Biomedicals
Supplier: MP Biomedicals
The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.
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Fixateur en solution dans gel polyacrylamide ( point isoélectrique ) Reag. Ph. Eur. 1138700
Supplier: VWR Chemicals
VWR®, Fixateur en solution dans gel polyacrylamide ( point isoélectrique )
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VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits
Supplier: VWR Chemicals
VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.
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Human cancer cell line blot
Supplier: G-Biosciences
Ready to screen human cancer cell line blots.
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Eye tissue region blot, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.
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Chemiluminescent acridinium esters
Supplier: ENZO LIFE SCIENCES
Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.
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Western blot assay kits, ScanLater™
Supplier: Molecular Devices
The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.
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Western ReProbe™ PLUS
Supplier: G-Biosciences
Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.
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Western blot stripping buffer, Western ReProbe™
Supplier: G-Biosciences
Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.
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Luminata™ Chemiluminescent ELISA HRP Substrates
Supplier: Merck
Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.
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Multiple tissue blot, single species
Supplier: G-Biosciences
These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.
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Chemicon® ReBlot Plus Mild Antibody Stripping Solution, 10X
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Visualizer and Visualizer EC Western Blot Detection Kits
Supplier: Merck
The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.
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Chemicon® ReBlot Western Blot Recycling Kit
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Brain tissue region blots, single species
Supplier: G-Biosciences
G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.
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Heart tissue region blots, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.
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Mouse single tissue blot, single species
Supplier: G-Biosciences
This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.
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Rat single tissue blot, single species
Supplier: G-Biosciences
This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.
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Nitrotyrosine BSA for immunoblotting experiments
Supplier: Cayman Chemical
This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.
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Western blots, pre-made, mouse subcellular fraction specific blot
Supplier: G-Biosciences
The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.
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Chemicon® ReBlot Plus Kits
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Luminata™ Western HRP Substrates Samples for Antibodies of All Affinities
Supplier: Merck
Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualisation, lipopolysaccharide analysis.
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Chemiluminescent detection reagents, Immobilon® Western HRP substrates
Supplier: Merck
Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.
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Single tissue blots, multiple species
Supplier: G-Biosciences
Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.
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Diluent for western blotting, Swift™ Western Diluent
Supplier: G-Biosciences
The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.