Vous avez recherché : Réactifs pour l'extraction des protéines
Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
SDS precipitation kit, SDS-Out™
Supplier: Thermo Fisher Scientific
SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
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Immobilised Pepsin
Supplier: G-Biosciences
A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
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Immobilised Papain
Supplier: G-Biosciences
A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
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Pierce™ Detergent Removal Spin Columns and Resin
Supplier: Thermo Fisher Scientific
Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.
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Immobilised papain, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
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Detergent removal spin column kit, HiPPR™
Supplier: Thermo Fisher Scientific
The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.
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Lysing matrix Z, MP Biomedicals
Supplier: MP Biomedicals
Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.
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Trypsin protease, MS grade
Supplier: Thermo Fisher Scientific
Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
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PopCulture® reagent
Supplier: Merck Millipore (Novagen)
Le réactif PopCulture® est un concentré à base de détergent qui peut être ajouté directement aux cultures d'E. coli pour une extraction efficace des protéines sans récolte de cellules. Les protéines recombinantes peuvent être directement criblées dans l'extrait brut ou purifiées par l'ajout d'une matrice d'affinité, le lavage du complexe matrice-protéines cibles afin d'en éliminer le milieu de culture usagé et les contaminants cellulaires et l'élution des protéines purifiées de la matrice. La procédure de purification de protéines PopCulture convient parfaitement au traitement robotisé haut débit d'échantillons pour la recherche protéomique ou pour toute autre application pouvant bénéficier de l'accélération et du confort accru des procédures.
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Endoproteinase Asp-N, excision grade, Pseudomonas fragi
Supplier: Merck Millipore (Calbiochem)
Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
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Endoproteinase Glu-C, excision grade, Staphylococcus aureus
Supplier: Merck Millipore (Calbiochem)
Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
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Protein extraction reagents, CytoBuster™
Supplier: Merck Millipore (Novagen)
The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.
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Master mix, BugBuster®
Supplier: Merck Millipore (Novagen)
BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.
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Washing solution, Protein-OUT™
Supplier: G-Biosciences
Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
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Uniform latex particles for immobilisation of biomolecules, PL-Latex
Supplier: VARIAN
Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.