Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.

Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MS(PEG)_n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)₁₂ is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

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Immobilised jacalin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

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Pierce™ Pegylation Reagents, Carboxy-PEG-Amine Compounds

Supplier: Thermo Fisher Scientific

The carboxy-PEG_n-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Recombinant streptavidin

Supplier: G-Biosciences

Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.

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Recombinant protein G, peroxidase conjugated, Calbiochem®

Supplier: Merck Millipore (Oncogene)

Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.

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TCEP, neutral, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

A ready-to-use stable, neutral form of the disulfide-reducing agent TCEP, hydrochloride.

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HCCA matrix

Supplier: BRUKER DALTRONIK

Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.

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BTSNM support materials, Chelex beads, 0,4 g with buffer

Supplier: G-Biosciences

Chelex beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

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Coupling catalyst, GlycoLink™

Supplier: Thermo Fisher Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

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Mass spectrometry reagents, MALDI matrices, single-use

Supplier: Thermo Fisher Scientific

MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.

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Pegylation reagent, MT(PEG)₄methyl-PEG-thiol compound

Supplier: Thermo Fisher Scientific

Methyl-PEG₄-thiol is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Pegylation reagent, CT(PEG)₁₂carboxy-PEG-thiol compound

Supplier: Thermo Fisher Scientific

Carboxy-PEG₁₂-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Pegylation reagents, methyl-PEG-amine compounds

Supplier: Thermo Fisher Scientific

The methyl-PEG_n-amine (MA[PEG]_n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

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Protein A from Staphylococcus aureus, MP Biomedicals

Supplier: MP Biomedicals

Protein A is a recombinant Fc-binding protein from Staphylococcus aureus, Cowan strain-1 gene. The product is a carboxy truncated material containing 301 amino acid residues. The non-binding regions have been removed to minimise potenial steric interference.