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The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
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6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Tampon de migration, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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SDS-Page running gel buffers, 10X concentrate
Supplier: Rockland Immunochemicals
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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SSC Solution tampon saline 20x concentrée (sodium citrate)
Supplier: G-Biosciences
Tampon d'hybridation, SSC Solution tampon saline 20x concentrée (sodium citrate)
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In-situ hybridisation buffer for HPV probes
Supplier: ENZO LIFE SCIENCES
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée pH 8,5
Supplier: PanReac AppliChem
Tampon de migration, Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée pH 8,5
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Electrophoresis running buffers
Supplier: G-Biosciences
There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.
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2X SDS-PAGE sample buffer without DTT or b-ME
Supplier: Rockland Immunochemicals
Sample buffer for protein electrophoresis.
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Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée
Supplier: Thermo Fisher Scientific
Tampon de migration, Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Tampon de migration, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, PBS (10X, Dulbecco's) powder pack
Supplier: PanReac AppliChem
Powder mixture to prepare PBS buffer (10X) according to Dulbecco.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline)
Supplier: G-Biosciences
The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4
Supplier: Cayman Chemical
250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: Rockland Immunochemicals
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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Blocking solution, BLOK™ Casein
Supplier: G-Biosciences
BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.
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Blocker™ Blocking Buffer, BLOTTO in TBS
Supplier: Thermo Fisher Scientific
Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.
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Blocking solution, BLOK™ BSA
Supplier: G-Biosciences
BLOK™ BSA is a 10% BSA protein blocking solution.
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Pierce™ Blocking Buffers, Protein Free
Supplier: Thermo Fisher Scientific
These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.
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SuperBlock™ Blocking Buffer
Supplier: Thermo Fisher Scientific
SuperBlock Blocking buffer is an optimised solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis. Blocking micro-plates, membranes, or tissues with a SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
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StartingBlock™ Blocking Buffer
Supplier: Thermo Fisher Scientific
These StartingBlock™ blocking buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.
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Blocker™ Blocking Buffer, Casein
Supplier: Thermo Fisher Scientific
Blocker™ Casein in PBS or TBS are ready-to-use, solutions of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.
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Non-animal sera protein blocking Agent, FISH-Blocker™
Supplier: G-Biosciences
FISH-Blocker™ uses a fish protein as the primary blocking agent to eliminate cross reactivity. Using a non mammalian protein eliminates or minimises the interaction of antibodies raised in mammals. It is offered either in TBS (Tris-buffered saline) or in PBS (phosphate-buffered saline) at pH 7,5.
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Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée
Supplier: OMEGA BIO-TEK
Tampon de migration, Tampon TAE 50x (Tris-acetate-EDTA), solution aqueuse 50x concentrée
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Pierce™ BupH™ Wash Buffers, Dry Blend Pack
Supplier: Thermo Fisher Scientific
BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
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Pierce™ Lane Marker Non-reducing Sample Buffer (5X)
Supplier: Thermo Fisher Scientific
Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.
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Wash buffer, Pierce™
Supplier: Thermo Fisher Scientific
These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.