Vous avez recherché : Protéines purification

Supplier: G-Biosciences

Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (Artocarpus integrifolia) seeds.

Supplier: ImmunoReagents

ImmunoReagents Bovine IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Bovine IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: ImmunoReagents

ImmunoReagents Donkey IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Donkey IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: G-Biosciences

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Supplier: G-Biosciences

This sulphhydryl coupling resin is designed for coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH). The iodoacetyl groups of the sulfhydryl coupling resin specifically react with free sulfhydryls to form covalent, permanent thioether bonds.

Supplier: AGILENT

Starter reagent kit for multiple affinity removal LC columns.

Supplier: G-Biosciences

Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Supplier: ImmunoReagents

ImmunoReagents Rabbit IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Rabbit IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: ImmunoReagents

ImmunoReagents Sheep IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Sheep IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Supplier: Biotium

TEV protease kit that may be used for cleaving proteins or peptides containing the TEV protease recognition sequence E-N-L-Y-F-Q↓(S/G/A/M/C/H).

Supplier: AGILENT

The affinity removal spin cartridge reagent kit is designed to remove the most interfering high-abundant protein (albumin) from human samples (such as plasma, serum, urine or cerebrospinal fluid).

Supplier: G-Biosciences

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Supplier: G-Biosciences

Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

Supplier: G-Biosciences

Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, E.coli, yeast, plant or as a control set that contains one 2 mg vial of each.

Supplier: Thermo Fisher Scientific

The Protein A IgG Purification Kit is useful for routine purification of antibodies. Samples containing IgG are incubated with immobilised Protein A in a buffer that facilitates binding. After non IgG components are washed from the column, the bound IgG is recovered by elution. The supplied buffers provide for maximum immunoglobulin binding and elution efficiency with the Protein A column.

Supplier: Thermo Fisher Scientific

The HisPur™ Ni-NTA Spin Purification Kit provides a fast, easy to use format for routine affinity purification of His-tagged fusion proteins and contains 0,2-ml HisPur™ Ni-NTA spin columns, as well as buffers required to purify polyhistidine-tagged proteins.

Supplier: MP Biomedicals

The FastPROTEIN™ Blue Matrix Kit (also known as Lysing Matrix B) is designed for rapid isolation and purification of protein from bacterial cultures.

Supplier: Thermo Fisher Scientific

Pierce™ Protein A agarose is a standard-capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Protein L Plus agarose is a high-capacity Protein L beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ protein G plus agarose is a high capacity protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Protein A/G agarose is a affinity resin for antibody purification.

Supplier: Thermo Fisher Scientific

The Pierce™ Melon™ Gel IgG Spin Purification Kit is a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Supplier: Thermo Fisher Scientific

Pierce™ Protein L agarose is a premium quality Protein L beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Glutathione Superflow Agarose consists of glutathione immobilised to a crosslinked 6% beaded agarose for purification of GST-tagged proteins.

Supplier: Thermo Fisher Scientific

Pierce™ Protein A Plus agarose is a high capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G plus agarose is a high capacity agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Immobilised D-Galactose agarose enables purification of lectins, galactosidases and other galactose-binding molecules. The immobilised D-galactose is a beaded agarose affinity resin that enables purification of agglutinins, lectins, toxins, and other galactose-binding proteins, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins.

Supplier: Thermo Fisher Scientific

NeutrAvidin™ UltraLink™ resin is a beaded polyacrylamide resin of immobilised NeutrAvidin™ Protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Cobalt Purification Kit is designed for the efficient purification of recombinant polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells.

Supplier: Thermo Fisher Scientific

AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.