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Protéines purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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Ion exchange chromatography media, Ion Exchanger I

Ion exchange chromatography media, Ion Exchanger I

Supplier: Merck

Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.

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J.T.Baker® BAKERBOND® PROchievA™, Résine de chromatographie d'affinité, Protéine recombinante A

J.T.Baker® BAKERBOND® PROchievA™, Résine de chromatographie d'affinité, Protéine recombinante A

Supplier: Avantor

L’appareil J.T.Baker® BAKERBOND® La résine de protéine A PROchievA™ offre une capacité de liaison dynamique supérieure avec une excellente stabilité alcaline liée au ligand propriétaire d'Avantor.

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Recombinant protein purification resins, IMAC

Recombinant protein purification resins, IMAC

Supplier: G-Biosciences

Cobalt chelating resin, for the purification of 6X His proteins

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Milieu pour chromatographie à interaction hydrophobe, J.T. Baker®, BAKERBOND®

Milieu pour chromatographie à interaction hydrophobe, J.T. Baker®, BAKERBOND®

Supplier: Avantor

Milieu à base de silice utilisé pour purifier les protéines à partir de l’hydrophobie induite par des sels différentiels. Le mode mixte permet une sélectivité unique avec son interaction hydrophobe et sa fonction d'échange d'ions.

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Protein purification kits, HOOK™

Protein purification kits, HOOK™

Supplier: G-Biosciences

For the rapid purification of soluble, 6X His or GST tagged proteins.

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Plaques de précipitation des protéines, J.T.Baker® BAKERBOND®

Plaques de précipitation des protéines, J.T.Baker® BAKERBOND®

Supplier: Avantor

Les plaques de précipitation des protéines J.T.Baker® BAKERBOND® permettent d'éliminer les protéines de manière rapide des échantillons biologiques.

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Amicon® Pro Purification System

Amicon® Pro Purification System

Supplier: Merck

The Amicon® Pro purification system - A centrifugal tool collapsing protein purifcation with concentration and buffer exchange.

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Immobilised Pepstatin for purification of Cathepsins/Pepsin/Pepstatin binding molecules

Immobilised Pepstatin for purification of Cathepsins/Pepsin/Pepstatin binding molecules

Supplier: G-Biosciences

Immobilised Pepstatin is for the purification of cathepsins, pepsin, bacterial aspartic proteases, HIV proteases and other molecules that bind pepstatin.

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Monoclonal IgG purification kit, Pearl™

Monoclonal IgG purification kit, Pearl™

Supplier: G-Biosciences

The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

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Protein purification kit, STRATEGY™

Protein purification kit, STRATEGY™

Supplier: G-Biosciences

STRATEGY™ is designed to allow researchers to rapidly screen a number of purification techniques in a small scale format to identify the system to isolate their protein of interest. The self-contained kit allows researchers to save time and money as separate buffers, resins and columns are not needed.

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Immobilised avidin for biotin purification

Immobilised avidin for biotin purification

Supplier: G-Biosciences

This avidin resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labelled antibodies.

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Affinity purification resins, anti-His, GenScript®

Supplier: Genscript

Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.

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Protein G

Protein G

Supplier: Rockland Immunochemicals

Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.

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Affinity purification resins, Glutathione Resin

Affinity purification resins, Glutathione Resin

Supplier: G-Biosciences

Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

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IgG purification kits, Pearl™

IgG purification kits, Pearl™

Supplier: G-Biosciences

The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

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Affinity chromatography resin, chelating nickel

Affinity chromatography resin, chelating nickel

Supplier: G-Biosciences

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

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IgG purification resin, Pearl™

Supplier: G-Biosciences

The Pearl™ IgG purification resin allows for the one-step purification of immunoglobulin G from serum. The resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

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Llama affinity purification resin

Llama affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Llama IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Llama IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Human affinity purification resin

Human affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Human IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Human IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Antibody clean up kit, Pearl™

Antibody clean up kit, Pearl™

Supplier: G-Biosciences

The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

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Goat affinity purification resin

Goat affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Goat IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Goat IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Protein A and G antibody purification kits

Protein A and G antibody purification kits

Supplier: G-Biosciences

Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

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Plaques d'extraction liquide, J.T.Baker® BAKERBOND®

Plaques d'extraction liquide, J.T.Baker® BAKERBOND®

Supplier: Avantor

Les plaques d’extraction liquide (SLE) J.T.Baker® BAKERBOND® constituent une alternative idéale et plus rapide aux méthodes d’extractions traditionnelles liquide-liquide, et elles permettent une récupération excellente des analytes des matrices biologiques.

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Mouse affinity purification resin

Mouse affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Mouse IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Mouse IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Proteasome Purification Kit

Supplier: ENZO LIFE SCIENCES

Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.

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Immobilised D-galactose for lectin purification

Immobilised D-galactose for lectin purification

Supplier: G-Biosciences

Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.

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Affinity purification Streptavidin Resin and buffers

Supplier: G-Biosciences

Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

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Chicken affinity purification resin

Chicken affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Chicken IgY Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Chicken IgY from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Protein A Sepharose™

Protein A Sepharose™

Supplier: Rockland Immunochemicals

Protein A Sepharose™ is a suspension of beads conjugated to native Protein A derived from Staphylococcus aureaus. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2 and rabbit.

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