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80 résultats pour Essais protéiniques

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Essais protéiniques

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

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Compat-Able™ Protein assay preparation reagent set

Compat-Able™ Protein assay preparation reagent set

Supplier: Thermo Fisher Scientific

The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

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Coomassie Plus™ protein assay kit, Compat-Able™

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

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Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™

Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™

Supplier: Thermo Fisher Scientific

Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.

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Coomassie (Bradford) protein assay kit, Pierce™

Coomassie (Bradford) protein assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.

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Portelite™ Fluorimetric Protein Quantitation Kit, Optimized for CytoCite™ and Qubit™ Fluorometers

Portelite™ Fluorimetric Protein Quantitation Kit, Optimized for CytoCite™ and Qubit™ Fluorometers

Supplier: AAT BIOQUEST

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

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Tetracysteine REASH Reagent *2 mM DMSO Solution*

Tetracysteine REASH Reagent *2 mM DMSO Solution*

Supplier: AAT BIOQUEST

REASH is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.

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Pierce™ Protease Assay Kits, Colorimetric and Fluorometric

Pierce™ Protease Assay Kits, Colorimetric and Fluorometric

Supplier: Thermo Fisher Scientific

Pierce™ Protease Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. These protease assays are also ideal for studying pH or temperature vs. activity profiles of purified proteases.

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Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible

Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible

Supplier: Thermo Fisher Scientific

This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

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Biotinylated-protein interaction pull down kit, Pierce™

Biotinylated-protein interaction pull down kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ biotinylated protein interaction pull-down kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

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Glutathione Fluorometric Assay Kit

Glutathione Fluorometric Assay Kit

Supplier: Abcam

Simple assay to detect total glutathione.

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Streptavidin poly-HRP conjugate, Pierce™

Streptavidin poly-HRP conjugate, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

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Non-interfering Protein Assay™ kit, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.

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Quantitative colorimetric peptide assay, Pierce™

Quantitative colorimetric peptide assay, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.

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Micro BCA protein assay kit

Micro BCA protein assay kit

Supplier: Boster Bio

The Micro BCA Protein Assay Kit is a ready to use, detergent-compatible, Western blot related, total protein analysis reagent used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs. concentration curve.

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Protein Assay, Protn-Latex™

Protein Assay, Protn-Latex™

Supplier: G-Biosciences

A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.

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Histone, from calf thymus, MP Biomedicals

Supplier: MP Biomedicals

Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.

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Kit 2-D Quant

Kit 2-D Quant

Supplier: Cytiva

Kit développé pour la détermination de protéines concentrées à partir de gels d'électrophorèse 2-D, SDS-PAGE ou IEF.

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Biotin labelling kit

Biotin labelling kit

Supplier: Abnova

Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.

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Protein quantitation kits, Upti-Light

Protein quantitation kits, Upti-Light

Supplier: Uptima

The BCA method is very sensitive for protein detection. Peptide bonds and the four amino acids cysteine, cystine , tryptophan and tyrosine are relevant to the determination. The formation of Cu (I) ions by proteins under alkaline conditions corresponds to the biuret method of determination. A Cu (I ) ion is then bound by two molecules of BCA to a red colour complex. This complex can be detected very sensitively.

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Soluble Collagen Type I Solution, Bovine

Soluble Collagen Type I Solution, Bovine

Supplier: MARKET SOURCE PART PROCESS

Soluble bovine type I collagen acid extracted (Telocollagen) at 5 mg/ml. Soluble collagen can be used as a thin coating for 2D cell culture in any cell culture dish. For use in 3D cell culture, collagen hydrogels, and bioinks, sterile 20 ml bottle.

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