You searched for: Essais protéiniques
Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.
Pierce™ Biotin Quantitation Kit
Supplier: Thermo Fisher Scientific
Pierce™ Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
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ReadiUse™ TCEP Removal Solution
Supplier: AAT BIOQUEST
TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins.
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Amplite® Rapid Colorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
Optimised to use with Nanodrop. Amplite™ colorimetric biotin quantitation kit is designed to quantify the number of biotins attached to a protein or other macromolecule with a nanodrop spectrometer.
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GST protein interaction pull down kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
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Octet® Anti-penta-HIS (HIS1K) biosensors
Supplier: Sartorius
For label-free quantitation and kinetic analysis of his-tagged proteins.
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Surface protein (allergen) test swab ALLTEC60, Clean-Trace™
Supplier: 3M Food Safety
3M™ Clean-Trace™ Surface Protein (Allergen) Test Swab ALLTEC60 is a rapid, simple, and reliable solution to monitor the presence of allergens on surfaces to effectively manage cleaning.
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Surface protein plus test swabs, PRO100, Clean-Trace™
Supplier: 3M Food Safety
3M™ Clean-Trace™ Surface Protein Plus Test Swab PRO100 is a simple, quick, and reliable method of checking for the removal of protein residues after cleaning and quickly identifies contamination.
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Bradford protein assay kits (B-Pak)
Supplier: OZ BIOSCIENCES
Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. This ready to use kit is provided with 1X reagent and 2 sets of BSA standards which means no dilution, filtration or calculation required. B-Pak is provided with adapted protocols for all experimental conditions – micro and macroassays.
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ReadiUse™ TCA Deproteinization Sample Preparation Kit
Supplier: AAT BIOQUEST
The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates.
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Tetracysteine FLASH Reagent *2 mM DMSO Solution*
Supplier: AAT BIOQUEST
FLASH is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.
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Lumiwox™ Acridinium NHS Ester
Supplier: AAT BIOQUEST
The acridinium NHS ester can be used to label proteins and nucleic acids.
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BCA protein assay kit (BCA-PAK)
Supplier: OZ BIOSCIENCES
BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content, even in the presence of detergents.
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Tetracysteine REASH Acetate *2 mM DMSO Solution*
Supplier: AAT BIOQUEST
REASH acetate is a cell-permeable version of REASH, which is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.
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Tetracysteine FLASH Diacetate *2 mM DMSO Solution*
Supplier: AAT BIOQUEST
FLASH diacetate is a cell-permeable version of FlASH, which is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.
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Solid phase biotinylation kits, EZ-Link™, Pierce™
Supplier: Thermo Fisher Scientific
This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.
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Pierce™ GlycoProtein Carbohydrate Estimation Kits
Supplier: Thermo Fisher Scientific
Pierce™ Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as a percentage of total purified protein mass.
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Pierce™ Polyhis-Protein Interaction Pull Down Kit
Supplier: Thermo Fisher Scientific
The Pierce™ his protein interaction pull-down kit contains the necessary components to capture and purify proteins that interact with his-tagged fusion proteins.
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Pierce™ Coomassie Plus (Bradford) Protein Assay Reagent
Supplier: Thermo Fisher Scientific
The bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ coomassie plus protein assay is a ready-to-use, reducing agent-compatible, improved bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.
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Pierce™ Coomassie Plus (Bradford) Protein Assay Kit
Supplier: Thermo Fisher Scientific
The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.
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Amplite® Fluorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.
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Phosphoprotein phosphate estimation assay Kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.
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Horseradish Peroxidase, Biotinylated, Pierce™
Supplier: Thermo Fisher Scientific
Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.
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BCA™ protein assay kits and reagents
Supplier: Thermo Fisher Scientific
Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
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Modified lowry protein assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.
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Portelite™ Rapid Fluorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
Optimised to use with CytoCite™ and Qubit™ fluorometers. Portelite™ rapid fluorimetric biotin quantitation kit is specifically optimised to quantify biotin and biotin conjugates with a portal fluorometer such as CytoCite™ and Qubit™ fluorometer.
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Portelite™ Fluorimetric Protein Quantitation Kit
Supplier: AAT BIOQUEST
Optimised for CytoCite™ and Qubit™ fluorometers. The Portelite™ fluorimetric protein quantitation kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays.
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Quantitative fluorometric peptide assay, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.
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Pierce™ Micro BCA™ Protein Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.
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Antibody labelling kits, HisTag® Antibody HRP Conjugate kit
Supplier: Merck Millipore (Novagen)
The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.
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Firefly luciferase flash assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.