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Tests cellulaires

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Kits d'analyse de viabilité cellulaire Calcein-AM

Kits d'analyse de viabilité cellulaire Calcein-AM

Supplier: Biotium

La calcéine AM est un composé perméable à la membrane non fluorescent. L’activité de l’estérase dans le cytoplasme de cellules viables convertit la calcéine AM en calcéine composée imperméable à la membrane de couleur vert fluorescent, qui est retenue dans les cellules viables à membranes plasmiques intactes. Le kit de test de viabilité / cytotoxicité pour cellules animales vivantes et mortes associe la calcéine AM au colorant essentiel éthidium homodimère III pour la quantification des cellules vivantes et mortes.

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Glucose (HK) assay kit

Supplier: Merck

The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.

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DuraClone Dry Unitized Antibody Panels For Flow Cytometry

DuraClone Dry Unitized Antibody Panels For Flow Cytometry

Supplier: Beckman Coulter

The dry DURAClone antibody panels are designed for clinical research studies. Every assay tube or well contains a layer with the dried-down antibody panel unitized for a single test.

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DNMT colorimetric drug discovery tests

DNMT colorimetric drug discovery tests

Supplier: ENZO LIFE SCIENCES

DNMT colorimetric drug discovery kit is a simple method to detect DNA methylation activity or inhibition.

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APO-BRDU™ kit, TUNEL Assay

Supplier: ENZO LIFE SCIENCES

Cellular assay, APO-BRDU™ kit

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Kits de test du calcium, kit de test FLIPR® Calcium 5

Kits de test du calcium, kit de test FLIPR® Calcium 5

Supplier: Molecular Devices

Le kit de test FLIPR® Calcium 5 fournit un test rapide, simple et fiable à base de fluorescence pour détecter les modifications du calcium intracellulaire.

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LEADING LIGHT® Wnt Reporter Assay Starter Kit

Supplier: ENZO LIFE SCIENCES

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

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Kits de test de caspase-3, NucView™ 488

Kits de test de caspase-3, NucView™ 488

Supplier: Biotium

Le substrat de caspase-3 NucView® 488 est un nouveau substrat de caspase cellulaire fluorogénique perméable à la membrane conçu pour détecter en temps réel l’activité de la caspase-3 dans les cellules vivantes.

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Kit de cardiotoxicité, EarlyTox™

Kit de cardiotoxicité, EarlyTox™

Supplier: Molecular Devices

Le kit de cardiotoxicité EarlyTox™ offre une méthode rapide, simple et fiable basée sur la fluorescence pour identifier des composés cardiotoxiques dans un test de biorélévation utilisant des cardiomyocytes dérivés de cellules souches.

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Hypoxia/oxidative stress detection kit, ROS-ID®

Supplier: ENZO LIFE SCIENCES

The ROS-ID® Hypoxia/Oxidative Stress Detection Kit is a specific detection system designed for functional detection of hypoxia and oxidative stress levels in live cells (both suspension and adherent) using fluorescent microscopy or flow cytometry.

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Apoptosis assay kit (Dual), NucView™ 488

Apoptosis assay kit (Dual), NucView™ 488

Supplier: Biotium

NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.

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OXI-TEK TBARS assay kit

OXI-TEK TBARS assay kit

Supplier: ENZO LIFE SCIENCES

The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.

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Calcium assay kits, Fura-2 QBT™ Calcium kit

Calcium assay kits, Fura-2 QBT™ Calcium kit

Supplier: Molecular Devices

The Fura-2 QBT™ Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2.

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Kits de test du calcium, kit de test FLIPR® Calcium 6 et 6-QF

Kits de test du calcium, kit de test FLIPR® Calcium 6 et 6-QF

Supplier: Molecular Devices

Les kits de test FLIPR® Calcium 6 et 6-QF fournissent un test rapide, simple et fiable à base de fluorescence pour détecter les modifications du calcium intracellulaire. Le kit de test Calcium 6 réduit considérablement le bruit de fond de fluorescence avec un protocole en une étape. Le kit de test Calcium 6-QF fournit une option neutre pour les cibles sensibles ou les applications de multiplexage.

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LDH Cytotoxicity WST Assay

Supplier: ENZO LIFE SCIENCES

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.

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Auto-Ubiquitinylation Kit

Auto-Ubiquitinylation Kit

Supplier: ENZO LIFE SCIENCES

This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.

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Autophagy detection tests, CYTO-ID®

Supplier: ENZO LIFE SCIENCES

CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.

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Cell viability assay kits, EarlyTox™ live/dead assay kits

Cell viability assay kits, EarlyTox™ live/dead assay kits

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Apoptosis assay kit, NucView® 488 and MitoView™ 633

Apoptosis assay kit, NucView® 488 and MitoView™ 633

Supplier: Biotium

NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

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Kit de test Steady-Luc™ Firefly HTS (lyophilisé)

Kit de test Steady-Luc™ Firefly HTS (lyophilisé)

Supplier: Biotium

La luciférine de luciole est largement utilisée comme gène rapporteur de transcription pour la recherche et le criblage de médicaments. Le kit Steady-Luc™ HTS peut être utilisé avec des lecteurs de plaque à luminescence sans systèmes d’injection d’échantillons ou pour le criblage à haut débit.

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Nuclear-ID™ red cell cycle kit, GFP-CERTIFIED®, for flow cytometry

Supplier: ENZO LIFE SCIENCES

GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry.

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Cell counting kit-F

Supplier: ENZO LIFE SCIENCES

Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages includes no radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay.

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Big blue

Supplier: AGILENT

These are used in all Big Blue rodents and cell lines.

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Caspase-3 cell assay kit

Supplier: ENZO LIFE SCIENCES

This kit is a complete assay system for measuring caspase-3 and caspase-3-like activity (DEVDase) in cellular extracts.

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Cellular senescence live cell analysis assay

Supplier: ENZO LIFE SCIENCES

Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.

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HAT activity assay kit

Supplier: ENZO LIFE SCIENCES

Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples.

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LEADING LIGHT® Wnt reporter cell line

Supplier: ENZO LIFE SCIENCES

Wnt reporter cell line for discovering Wnt pathway modulators.

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LEADING LIGHT® Wnt cell line assay reagents

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit

Supplier: ENZO LIFE SCIENCES

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

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