Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Kits d'analyse de viabilité cellulaire Calcein-AM
Supplier: Biotium
La calcéine AM est un composé perméable à la membrane non fluorescent. L’activité de l’estérase dans le cytoplasme de cellules viables convertit la calcéine AM en calcéine composée imperméable à la membrane de couleur vert fluorescent, qui est retenue dans les cellules viables à membranes plasmiques intactes. Le kit de test de viabilité / cytotoxicité pour cellules animales vivantes et mortes associe la calcéine AM au colorant essentiel éthidium homodimère III pour la quantification des cellules vivantes et mortes.
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Glucose (HK) assay kit
Supplier: Merck
The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.
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DuraClone Dry Unitized Antibody Panels For Flow Cytometry
Supplier: Beckman Coulter
The dry DURAClone antibody panels are designed for clinical research studies. Every assay tube or well contains a layer with the dried-down antibody panel unitized for a single test.
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DNMT colorimetric drug discovery tests
Supplier: ENZO LIFE SCIENCES
DNMT colorimetric drug discovery kit is a simple method to detect DNA methylation activity or inhibition.
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Kits de test du calcium, kit de test FLIPR® Calcium 5
Supplier: Molecular Devices
Le kit de test FLIPR® Calcium 5 fournit un test rapide, simple et fiable à base de fluorescence pour détecter les modifications du calcium intracellulaire.
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LEADING LIGHT® Wnt Reporter Assay Starter Kit
Supplier: ENZO LIFE SCIENCES
The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.
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Kits de test de caspase-3, NucView™ 488
Supplier: Biotium
Le substrat de caspase-3 NucView® 488 est un nouveau substrat de caspase cellulaire fluorogénique perméable à la membrane conçu pour détecter en temps réel l’activité de la caspase-3 dans les cellules vivantes.
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Kit de cardiotoxicité, EarlyTox™
Supplier: Molecular Devices
Le kit de cardiotoxicité EarlyTox™ offre une méthode rapide, simple et fiable basée sur la fluorescence pour identifier des composés cardiotoxiques dans un test de biorélévation utilisant des cardiomyocytes dérivés de cellules souches.
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Hypoxia/oxidative stress detection kit, ROS-ID®
Supplier: ENZO LIFE SCIENCES
The ROS-ID® Hypoxia/Oxidative Stress Detection Kit is a specific detection system designed for functional detection of hypoxia and oxidative stress levels in live cells (both suspension and adherent) using fluorescent microscopy or flow cytometry.
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Apoptosis assay kit (Dual), NucView™ 488
Supplier: Biotium
NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.
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OXI-TEK TBARS assay kit
Supplier: ENZO LIFE SCIENCES
The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.
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Calcium assay kits, Fura-2 QBT™ Calcium kit
Supplier: Molecular Devices
The Fura-2 QBT™ Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2.
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Kits de test du calcium, kit de test FLIPR® Calcium 6 et 6-QF
Supplier: Molecular Devices
Les kits de test FLIPR® Calcium 6 et 6-QF fournissent un test rapide, simple et fiable à base de fluorescence pour détecter les modifications du calcium intracellulaire. Le kit de test Calcium 6 réduit considérablement le bruit de fond de fluorescence avec un protocole en une étape. Le kit de test Calcium 6-QF fournit une option neutre pour les cibles sensibles ou les applications de multiplexage.
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LDH Cytotoxicity WST Assay
Supplier: ENZO LIFE SCIENCES
Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.
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Auto-Ubiquitinylation Kit
Supplier: ENZO LIFE SCIENCES
This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.
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Autophagy detection tests, CYTO-ID®
Supplier: ENZO LIFE SCIENCES
CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.
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Cell viability assay kits, EarlyTox™ live/dead assay kits
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Apoptosis assay kit, NucView® 488 and MitoView™ 633
Supplier: Biotium
NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.
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Kit de test Steady-Luc™ Firefly HTS (lyophilisé)
Supplier: Biotium
La luciférine de luciole est largement utilisée comme gène rapporteur de transcription pour la recherche et le criblage de médicaments. Le kit Steady-Luc™ HTS peut être utilisé avec des lecteurs de plaque à luminescence sans systèmes d’injection d’échantillons ou pour le criblage à haut débit.
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Nuclear-ID™ red cell cycle kit, GFP-CERTIFIED®, for flow cytometry
Supplier: ENZO LIFE SCIENCES
GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry.
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Cell counting kit-F
Supplier: ENZO LIFE SCIENCES
Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages includes no radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay.
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Caspase-3 cell assay kit
Supplier: ENZO LIFE SCIENCES
This kit is a complete assay system for measuring caspase-3 and caspase-3-like activity (DEVDase) in cellular extracts.
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Cellular senescence live cell analysis assay
Supplier: ENZO LIFE SCIENCES
Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.
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HAT activity assay kit
Supplier: ENZO LIFE SCIENCES
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples.
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LEADING LIGHT® Wnt reporter cell line
Supplier: ENZO LIFE SCIENCES
Wnt reporter cell line for discovering Wnt pathway modulators.
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LEADING LIGHT® Wnt cell line assay reagents
Supplier: ENZO LIFE SCIENCES
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
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ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit
Supplier: ENZO LIFE SCIENCES
Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.