Tests cellulaires
Les essais cellulaires ont toujours été un outil puissant dans le laboratoire de recherche. Ils sont extrêmement polyvalents et peuvent être conçus pour mesurer pratiquement toute fonction cellulaire ou biochimique. La détection sensible de sondes fluorescentes aide à évaluer la signalisation cellulaire et les voies de mort cellulaire, ce qui est utile pour la quantification rapide de la viabilité cellulaire. Les essais cellulaires peuvent être utilisés pour mesurer les effets de différents traitements sur la viabilité cellulaire et pour évaluer les effets toxiques médiés par divers mécanismes.
Neurotransmitter transporter uptake assay kits
Supplier: Molecular Devices
The Neurotransmitter Transporter Uptake Assay Kit provides a fast, simple and reliable fluorescence-based assay for the detection of dopamine, norepinephrine and serotonin transporter (SERT, NET and DAT, respectively) activity in expressing cells.
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FLUOR DE LYS® HDAC fluorometric cellular activity assay kit
Supplier: Enzo Life Sciences
The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
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TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit
Supplier: Enzo Life Sciences
The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.
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Mitochondria/cytosol fractionation kit
Supplier: Enzo Life Sciences
For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.
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Myeloperoxidase fluorometric detection kit
Supplier: Enzo Life Sciences
The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.
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Triglyceride colorimetric assay kit
Supplier: Cayman Chemical
Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.
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QBT™ Fatty acid uptake assay kit
Supplier: Molecular Devices
QBT™ Fatty Acid Uptake Assay Kit is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.
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Cell viability assay kits, EarlyTox™ live/dead assay kits
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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EZAssay™ TBARS Estimation Kit for Lipid Peroxidation
Supplier: HIMEDIA
Cellular assay, EZAssay™ TBARS estimation kit for lipid peroxidation
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.
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Neutrophil/Monocyte Respiratory Burst Assay Kit
Supplier: Cayman Chemical
Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.
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TUNEL assay kits
Supplier: Biotium
TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.
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LEADING LIGHT® Wnt cell line medium pack
Supplier: Enzo Life Sciences
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
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Kits de marquage de membranes cytoplasmiques, CellBrite™
Supplier: Biotium
Les kits CellBrite sont conçus pour marquer les membranes cytoplasmiques cellulaires avec un colorant membranaire fluorescent préalablement dissous en tant que solution de coloration optimisée. En combinant des colorants membranaires CellBrite, il est possible de marquer plusieurs populations de cellules avec des couleurs distinctives, une capacité utile pour les études d’interactions cellule-cellule.
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HIF-1α transcription factor assay Kit
Supplier: Cayman Chemical
HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.
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