Vous avez recherché : Systèmes de liaison et de marquage des protéines

Supplier: Biotium

CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.

Supplier: Biotium

The ExoBrite™ EV Surface Stain Sampler Kit, Green was developed to offer each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest.

Supplier: ENZO LIFE SCIENCES

Nucleus dye

Supplier: ENZO LIFE SCIENCES

Cell viability dye

Supplier: ENZO LIFE SCIENCES

The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

Supplier: ENZO LIFE SCIENCES

Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labelling.

Supplier: ENZO LIFE SCIENCES

Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Aqua 431 dUTP can also be used for multicolor fluorescence labelling.

Supplier: ENZO LIFE SCIENCES

A complete kit for the labeling of antibodies, proteins, peptides, and nucleic acids with Acridinium NHS Ester.

Supplier: Biotium

CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

Supplier: Biotium

Mix-n-Stain™ antibody labelling kits utilise a technology that allows rapid antibody labelling without a purification step.

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling of nanobodies with fluorescent CF® dyes or biotin.

Supplier: Biotium

Biotium propose un certain nombre de formats de colorants réactifs pour le marquage de protéines, d'acides nucléiques et d'autres biomolécules avec des colorants fluorescents. Les colorants CF® offrent des avantages en termes de luminosité et de photostabilité par rapport à d'autres colorants commerciaux similaires. Les colorants CF® sont disponibles dans un certain nombre de formats de colorants réactifs. Les couleurs des colorants CF® couvrent les spectres visible et proche infrarouge.

Supplier: Biotium

Reactive dyes containing an amine functional group.

Supplier: Biotium

CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.

Supplier: AGILENT

Luminescent peel-off stickers are used for accurately marking and aligning autoradiographs.

Supplier: Biotium

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

Supplier: Rockland Immunochemicals

Peroxidase anti-peroxidase soluble complexes are used for peroxidase enzymatic labelling experiments.

Supplier: G-Biosciences

SDC™ (Steroid/Drug/Compound) Immobilisation Kit is designed for the immobilisation of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The kit uses Immobilised DADPA (diaminodipropylamine) resin to bind steroids, drugs and chemicals through their active hydrogens.

Supplier: Biotium

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.

Supplier: Biotium

Streptavidin conjugates can be used to detect biotinylated molecules with extremely high sensitivity. They are commonly used as secondary reagents to detect biotinylated primary probes such as antibodies in flow cytometry, Western blotting, immunocytochemistry and other applications.

Supplier: Biotium

CF® Dye alkynes react with azide groups via copper-catalysed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.

Supplier: Apollo Scientific

Liquid product orange to red odourless pH 6,4 to 6,6. Stabiliser for conjugates with monomeric HRP. Preservation of very low conjugate concentrations.

Supplier: Apollo Scientific

Liquid product colourless to slightly yellow or greenish odourless pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for controls and protein standards.

Supplier: Biotium

Luciferin is a substrate for the enzyme firefly luciferase.

Supplier: ENZO LIFE SCIENCES

The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

Supplier: ENZO LIFE SCIENCES

Improved data quality and analysis results through greater biotin incorporation.

Supplier: ENZO LIFE SCIENCES

Gold 525 [5(6)-Carboxyrhodamine 6G] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Gold 525 dUTP can also be used for multicolor fluorescence labelling.

Supplier: ENZO LIFE SCIENCES

Red 580 dUTP (lyophilised)

Supplier: Thermo Fisher Scientific

Pierce™ Hydrazide-activated strepavidin conjugate includes recombinant streptavidin in a purified form activated for crosslinking to carbonyl groups in a molecule.

Supplier: Thermo Fisher Scientific

Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.