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364 résultats pour Systèmes de liaison et de marquage des protéines

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Systèmes de liaison et de marquage des protéines

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

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HRP - conjugate stabiliser, SeramunStab® STC

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.

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Biotin reagents, OneQuant™ HOOK™

Supplier: G-Biosciences

The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.

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Nuclear-ID™ Blue/Red cell viability reagent (GFP-Certified™)

Supplier: ENZO LIFE SCIENCES

Cell viability dye

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CF™ azide reactive dyes

Supplier: Biotium

CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

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femtoCHROMO™- HRP Substrate for chromogenic detection

femtoCHROMO™- HRP Substrate for chromogenic detection

Supplier: G-Biosciences

A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.

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Wheat germ agglutinin (WGA) conjugates

Wheat germ agglutinin (WGA) conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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Gold 550 dUTP

Supplier: ENZO LIFE SCIENCES

Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analogue can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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Fluorescein-12-ddUTP

Fluorescein-12-ddUTP

Supplier: ENZO LIFE SCIENCES

Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E.coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labelled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.

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Bio-16-ddUTP, tetralithium salt

Bio-16-ddUTP, tetralithium salt

Supplier: ENZO LIFE SCIENCES

Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.

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Gold 525 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Gold 525 dUTP (lyophilised)

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Organelle-ID™ RGB reagent II

Supplier: ENZO LIFE SCIENCES

Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids

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ExoBrite™ True EV Membrane Stains

Supplier: Biotium

Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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Antibody labelling kits, Mix-n-Stain™ FITC

Antibody labelling kits, Mix-n-Stain™ FITC

Supplier: Biotium

Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.

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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates

Supplier: Biotium

CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.

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HRP-conjugate stabiliser, SeramunStab® STA2

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.

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CF® Dye cAMP

Supplier: Biotium

Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.

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Tyramide amplification buffer plus

Tyramide amplification buffer plus

Supplier: Biotium

An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

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R-Phycoerythrin (RPE)

R-Phycoerythrin (RPE)

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin.

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Gold 525 dUTP

Supplier: ENZO LIFE SCIENCES

Gold 525 [5(6)-Carboxyrhodamine 6G] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Gold 525 dUTP can also be used for multicolor fluorescence labelling.

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Red 580 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Red 580 dUTP (lyophilised)

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BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System

Supplier: ENZO LIFE SCIENCES

The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

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100-reaction single-round RNA amplification and Biotin labeling system

Supplier: ENZO LIFE SCIENCES

Improved data quality and analysis results through greater biotin incorporation.

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Nanobody labelling kits, Mix-n-Stain™

Nanobody labelling kits, Mix-n-Stain™

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling of nanobodies with fluorescent CF® dyes or biotin.

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Colorants réactifs CF® et kits de marquage de protéines

Colorants réactifs CF® et kits de marquage de protéines

Supplier: Biotium

Biotium propose un certain nombre de formats de colorants réactifs pour le marquage de protéines, d'acides nucléiques et d'autres biomolécules avec des colorants fluorescents. Les colorants CF® offrent des avantages en termes de luminosité et de photostabilité par rapport à d'autres colorants commerciaux similaires. Les colorants CF® sont disponibles dans un certain nombre de formats de colorants réactifs. Les couleurs des colorants CF® couvrent les spectres visible et proche infrarouge.

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CF®DI Hydrazide

CF®DI Hydrazide

Supplier: Biotium

CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.

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Peroxidase anti-peroxidase (PAP) soluble complexes

Supplier: Rockland Immunochemicals

Peroxidase anti-peroxidase soluble complexes are used for peroxidase enzymatic labelling experiments.

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Immobilisation kit, SDC™ (Steroid/Drug/Compound)

Immobilisation kit, SDC™ (Steroid/Drug/Compound)

Supplier: G-Biosciences

SDC™ (Steroid/Drug/Compound) Immobilisation Kit is designed for the immobilisation of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The kit uses Immobilised DADPA (diaminodipropylamine) resin to bind steroids, drugs and chemicals through their active hydrogens.

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Reactive dye conjugates

Supplier: Biotium

Reactive dyes containing an amine functional group.

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D-luciferin luminescent substrates

Supplier: Biotium

Luciferin is a substrate for the enzyme firefly luciferase.

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