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Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.
Antibody fab fragmentation kit
Supplier: G-Biosciences
Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.
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Antibody F(ab)₂ fragmentation kits
Supplier: G-Biosciences
The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
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Kits d'extraction de protéines, BugBuster® Plus Lysonase™, Novagen®
Supplier: Merck Millipore (Novagen)
Le kit BugBuster® Plus Lysonase™ inclut le réactif d'extraction de protéines BugBuster et le réactif de biotraitement Lysonase, un mélange optimisé et prêt à l'emploi de rLysozyme™ et de nucléase Benzonase®. Cette puissante association de deux réactifs permet une récupération maximale des protéines solubles actives dans les bactéries à Gram négatif et à Gram positif. Les réactifs BugBuster et rLysozyme lysent efficacement les cellules bactériennes tandis que la Benzonase dégrade les acides nucléiques pour faciliter le traitement en aval. Requiert 5 ml de BugBuster et 10 µl de Lysonase par gramme de pâte cellulaire. Les kits fournissent suffisamment de réactifs pour l'extraction de protéines dans 20 ou 100 g de pâte cellulaire.
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Detergent removal system, DetergentOUT™ GBS10
Supplier: G-Biosciences
Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.
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Pierce™, Detoxi-Gel™ Endotoxin Removing Columns and Gel
Supplier: Thermo Fisher Scientific
Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.
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Phosphoprotein enrichment kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.
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Carrier proteins, Imject™ Blue Carrier™
Supplier: Thermo Fisher Scientific
Imject™ Blue Carrier™ protein is a highly soluble, mollusc-derived hemocyanin that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.
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FOCUS™ Protein Alkylation Kit
Supplier: G-Biosciences
The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
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Carrier proteins, maleimide-activated carrier proteins, Imject™ Pierce™
Supplier: Thermo Fisher Scientific
Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.
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Mitochondria isolation kit for tissue
Supplier: Thermo Fisher Scientific
The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.
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InGel™ Blue for protein digestion
Supplier: G-Biosciences
InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
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Protein quantitation reagent, TMTzero™ label reagent
Supplier: Thermo Fisher Scientific
Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
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Fluor-de-Lys® Developer I concentrate
Supplier: ENZO LIFE SCIENCES
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
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Protein fractionation kit, FOCUS™ cytoplasmic and nuclear proteins
Supplier: G-Biosciences
FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.
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Glycoprotein Isolation Kit, Cona And WGA, Pierce™
Supplier: Thermo Fisher Scientific
The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.
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Pierce™ In-solution Tryptic Digestion and Guanidination Kit
Supplier: Thermo Fisher Scientific
The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
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In-Gel tryptic digestion kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
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Albumin/IgG removal kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
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Albumin depletion kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Albumin Depletion Kit improves serum component analysis by rapidly removing abundant albumin protein from serum samples. The albumin depletion resin provided in the kit is a high capacity, immobilised Cibacron Blue dye agarose resin, which binds a variety of species-specific albumins.
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FOCUS™ protein reduction-alkylation kit
Supplier: G-Biosciences
The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.
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FOCUS™ protein reductant kit
Supplier: G-Biosciences
FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
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Ubiquitin enrichment kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
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Detergent removal system, DetergentOUT™ Tween®
Supplier: G-Biosciences
DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.
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Carrier proteins
Supplier: G-Biosciences
The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.
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SilverOUT™, G-Biosciences
Supplier: G-Biosciences
SilverOUT removes interfering silver ions from gel pieces.
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Biotin resin
Supplier: G-Biosciences
Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
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Antigen retrieval reagent, IHC
Supplier: ENZO LIFE SCIENCES
Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.
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Immobilised Ficin
Supplier: G-Biosciences
Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
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Optimizer blueBALLS™
Supplier: G-Biosciences
The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
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InGel™ for protein digestion
Supplier: G-Biosciences
InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.