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108 résultats pour Protéines extraction

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Protéines extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

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Antibody F(ab)₂ fragmentation kits

Antibody F(ab)₂ fragmentation kits

Supplier: G-Biosciences

The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

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Protein quantitation kit, AccuOrange™

Protein quantitation kit, AccuOrange™

Supplier: Biotium

AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).

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Kits d'extraction de protéines, BugBuster® Plus Lysonase™, Novagen®

Supplier: Merck Millipore (Novagen)

Le kit BugBuster® Plus Lysonase™ inclut le réactif d'extraction de protéines BugBuster et le réactif de biotraitement Lysonase, un mélange optimisé et prêt à l'emploi de rLysozyme™ et de nucléase Benzonase®. Cette puissante association de deux réactifs permet une récupération maximale des protéines solubles actives dans les bactéries à Gram négatif et à Gram positif. Les réactifs BugBuster et rLysozyme lysent efficacement les cellules bactériennes tandis que la Benzonase dégrade les acides nucléiques pour faciliter le traitement en aval. Requiert 5 ml de BugBuster et 10 µl de Lysonase par gramme de pâte cellulaire. Les kits fournissent suffisamment de réactifs pour l'extraction de protéines dans 20 ou 100 g de pâte cellulaire.

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Detergent removal system, DetergentOUT™ GBS10

Detergent removal system, DetergentOUT™ GBS10

Supplier: G-Biosciences

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

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Pierce™, Detoxi-Gel™ Endotoxin Removing Columns and Gel

Pierce™, Detoxi-Gel™ Endotoxin Removing Columns and Gel

Supplier: Thermo Fisher Scientific

Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

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Phosphoprotein enrichment kit, Pierce™

Phosphoprotein enrichment kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.

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Carrier proteins, Imject™ Blue Carrier™

Supplier: Thermo Fisher Scientific

Imject™ Blue Carrier™ protein is a highly soluble, mollusc-derived hemocyanin that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.

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FOCUS™ Protein Alkylation Kit

FOCUS™ Protein Alkylation Kit

Supplier: G-Biosciences

The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.

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Carrier proteins, maleimide-activated carrier proteins, Imject™ Pierce™

Carrier proteins, maleimide-activated carrier proteins, Imject™ Pierce™

Supplier: Thermo Fisher Scientific

Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.

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Mitochondria isolation kit for tissue

Mitochondria isolation kit for tissue

Supplier: Thermo Fisher Scientific

The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.

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InGel™ Blue for protein digestion

InGel™ Blue for protein digestion

Supplier: G-Biosciences

InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

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Protein quantitation reagent, TMTzero™ label reagent

Protein quantitation reagent, TMTzero™ label reagent

Supplier: Thermo Fisher Scientific

Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.

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Fluor-de-Lys® Developer I concentrate

Supplier: ENZO LIFE SCIENCES

FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).

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FOCUS™ protein reduction-alkylation kit

FOCUS™ protein reduction-alkylation kit

Supplier: G-Biosciences

The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.

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FOCUS™ protein reductant kit

FOCUS™ protein reductant kit

Supplier: G-Biosciences

FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.

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Ubiquitin enrichment kit, Pierce™

Ubiquitin enrichment kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.

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Pierce™ In-solution Tryptic Digestion and Guanidination Kit

Pierce™ In-solution Tryptic Digestion and Guanidination Kit

Supplier: Thermo Fisher Scientific

The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.

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In-Gel tryptic digestion kit, Pierce™

In-Gel tryptic digestion kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

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Albumin/IgG removal kit, Pierce™

Albumin/IgG removal kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

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Albumin depletion kit, Pierce™

Albumin depletion kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Albumin Depletion Kit improves serum component analysis by rapidly removing abundant albumin protein from serum samples. The albumin depletion resin provided in the kit is a high capacity, immobilised Cibacron Blue dye agarose resin, which binds a variety of species-specific albumins.

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InGel™ for protein digestion

InGel™ for protein digestion

Supplier: G-Biosciences

InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

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Protein fractionation kit, FOCUS™ cytoplasmic and nuclear proteins

Protein fractionation kit, FOCUS™ cytoplasmic and nuclear proteins

Supplier: G-Biosciences

FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.

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Protein reducing agents

Protein reducing agents

Supplier: G-Biosciences

Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

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Glycoprotein Isolation Kit, Cona And WGA, Pierce™

Glycoprotein Isolation Kit, Cona And WGA, Pierce™

Supplier: Thermo Fisher Scientific

The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

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SilverOUT™, G-Biosciences

SilverOUT™, G-Biosciences

Supplier: G-Biosciences

SilverOUT removes interfering silver ions from gel pieces.

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Biotin resin

Biotin resin

Supplier: G-Biosciences

Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.

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Antigen retrieval reagent, IHC

Supplier: ENZO LIFE SCIENCES

Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

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Immobilised Ficin

Immobilised Ficin

Supplier: G-Biosciences

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

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Optimizer blueBALLS™

Optimizer blueBALLS™

Supplier: G-Biosciences

The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.

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Organelle enrichment kits

Organelle enrichment kits

Supplier: Thermo Fisher Scientific

Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.

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