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Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.
Bacterial Protein Extraction Kits, B-PER™
Supplier: Thermo Fisher Scientific
B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).
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Complete bacterial protein extraction reagent, B-PER™
Supplier: Thermo Fisher Scientific
B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
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Total protein extraction kit, TPE™
Supplier: G-Biosciences
Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.
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Membrane protein extraction kit, Pierce™ Mem-PER™ Plus
Supplier: Thermo Fisher Scientific
The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
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Proteome kits, mammalian proteome, FOCUS™
Supplier: G-Biosciences
Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
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Protein extraction kits, ProteoExtract™
Supplier: Merck Millipore (Calbiochem)
ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.
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Proteome kits, FOCUS™ Bacterial Proteome
Supplier: G-Biosciences
Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from E.coli, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
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FOCUS™ Yeast proteome kits
Supplier: G-Biosciences
Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
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Proteome kits, FOCUS™ Insect Proteome
Supplier: G-Biosciences
Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.
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Protein fractionation kit, FOCUS™ Global Fractionation
Supplier: G-Biosciences
FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
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Mouse embryonic fibroblast isolation kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
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Protein fractionation kit, FOCUS™ soluble and insoluble
Supplier: G-Biosciences
FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.
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Proteome kits, FOCUS™ Plant Proteome
Supplier: G-Biosciences
Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.
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Protein fractionation kit, nuclear and cytoplasmic extraction kit
Supplier: G-Biosciences
The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.
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Isobaric mass tagging kits and reagent sets, TMT™
Supplier: Thermo Fisher Scientific
Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.
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Protein extraction reagents, CytoBuster™
Supplier: Merck Millipore (Novagen)
The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.
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Albumin removal kit, AlbuminOUT™
Supplier: G-Biosciences
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.
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Protein fractionation kit, FOCUS™ mitochondria
Supplier: G-Biosciences
FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
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Protein fractionation kit, FOCUS™ Membrane Proteins
Supplier: G-Biosciences
FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.
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EpiXtract® total histone extraction kit (high throughput)
Supplier: ENZO LIFE SCIENCES
Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.
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EpiXtract® Nuclear protein isolation kit II (nucleic acid-free)
Supplier: ENZO LIFE SCIENCES
Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.
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FOCUS™ SubCell, protein fractionation kit
Supplier: G-Biosciences
FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.
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Protein fractionation kit, FOCUS™ signal proteins
Supplier: G-Biosciences
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
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Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits
Supplier: G-Biosciences
Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG1 Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.
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EpiXtract® total histone extraction kit
Supplier: ENZO LIFE SCIENCES
Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.
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Protein fractionation kit, FOCUS™ Glycoprotein
Supplier: G-Biosciences
FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.
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Protein quantitation kit, AccuOrange™
Supplier: Biotium
AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).
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EpiXtract® Nuclear protein isolation kit
Supplier: ENZO LIFE SCIENCES
Rapid, reliable method to isolate nuclear proteins in mammalian cells and tissues.
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SDS detection and estimation reagent kit
Supplier: G-Biosciences
A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.
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Protein enrichment kit, FOCUS™ PhosphoRich™
Supplier: G-Biosciences
FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUS™ PhosphoRich™ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.