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Tampons

VWR propose un éventail de solutions tampons pour répondre aux besoins spécifiques des procédures chimiques et microbiologiques en laboratoires. Pour isoler et séparer les protéines lors du processus d'électrophorèse, l'utilisateur pourra sélectionner des tampons en poudre ou liquides qui protègent et suivent les échantillons protéiniques durant le process. Une large gamme de tampons de pH, de compositions et de formats différents est proposée pour la calibration et la normalisation des pH mètres. Pour la purification et la récupération des protéines dans des procédés de chromatographie, sélectionnez des tampons dans des formats adéquats aux procédures d’échange d’ions. La plupart des tampons sont disponibles en concentrés liquides, dans des bouteilles prêtes à l’emploi ou dans des formats déshydratés.

2-D Protein Extraction Buffer

2-D Protein Extraction Buffer

Supplier: Cytiva

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.

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Buffer solutions as per DIN 19266-01: 2000

Buffer solutions as per DIN 19266-01: 2000

Supplier: WTW

These standard buffer solutions (work reference buffer solutions) are widely used in precision applications.

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Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, without calcium, without magnesium

Supplier: Thermo Fisher Scientific

Cell culture buffer, DPBS, 1X

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Phosphate-buffered saline (PBS, 10X), RNase free

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X, RNAse free

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EDTA disodium salt solution, 0,2% in phosphate buffered saline, MP Biomedicals

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

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TE buffer, pH 8,1, RNAse free

Supplier: Thermo Fisher Scientific

Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.

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Phosphate-buffered saline (PBS, 10X), pH 7,6

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X

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Sodium bicarbonate, Corning®

Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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Krebs-Ringer solution, bicarbonate-buffered

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

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His·Bind® buffer kit, Novagen®

Supplier: Merck Millipore (Novagen)

The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

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Carbonate-buffered saline (5X), pH 9,5

Supplier: Thermo Fisher Scientific

Cell culture buffer, Carbonate-buffered Salin, 5X

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His buffer kit

His buffer kit

Supplier: Cytiva

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.

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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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Sodium acetate, Corning® 3M™

Sodium acetate, Corning® 3M™

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

Supplier: Thermo Fisher Scientific

Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

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10% SDS (Sodium Dodecyl Sulfate), Corning®

10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

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Fluoride buffer solution According to DEV D4, Fluka™

Supplier: Honeywell Chemicals

TISAB according to DEV D4.

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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Buffer capsules, coloured coded

Supplier: REAGECON

pH buffer capsules, reference standard, Valeur pH: 10,00, ±0,02, Température de réference: 25 °C, bleu

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Buffer kit for antibody purification on protein A column

Supplier: Thermo Fisher Scientific

Buffer kit for antibody purification

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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Used to minimise cell attachment or aggregation.

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pH buffer solutions, colour coded

Supplier: REAGECON

Colour coded pH buffer solutions for the calibration of pH meters.

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Carbonate Borax Free Buffer Solution

Supplier: REAGECON

This range of Reagecon pH Buffers are sodium tetraborate (Borax) free. They are not subject to classification by EU regulatory authorities as toxic to reproduction under CLP category 1B. Similar in approach to the existing range of Reagecon Mercury Free Buffers, these Buffer Solutions have been developed to enhance user choice and allay safety concerns, without any impact on fitness for purpose or product performance.

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Buffer solutions

Buffer solutions

Supplier: REAGECON

Buffer solution, Valeur pH: 10,00, ±0,01, Température de réference: 20 °C, Bouteille en plastique

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Hank's balanced salt solution, modified, MP Biomedicals

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Supplier: MP Biomedicals

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.

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Hank's balanced salts, MP Biomedicals

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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Ab Buffer Kit

Ab Buffer Kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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pH buffers

pH buffers

Supplier: Cole-Parmer

Available as single use sachets or as 500 ml bottles.

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