Vous avez recherché : End-point PCR Enzymes and Kits

Supplier: FINNZYMES REAGENTS

Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Supplier: FINNZYMES REAGENTS

Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli.

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli. Native Taq DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

Supplier: MP Biomedicals

Taq DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

Supplier: Cytiva

PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: Merck Millipore (Novagen)

L'ADN polymérase KOD HiFi est une enzyme de correction unique, isolé de l'hyperthermophile Thermococcus kodakaraensis KOD1, qui possède une processivité et une fidélité supérieures permettant une amplification PCR plus rapide et plus précise que les enzymes conventionnels.

Supplier: ENZO LIFE SCIENCES

Optimised standard PCR for enhanced speed, yield, and specificity.

Supplier: AGILENT

High-quality polymerases for difficult to amplify DNA targets.

Supplier: AGILENT

Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Supplier: ENZO LIFE SCIENCES

Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.

Supplier: AGILENT

Detects low copy number target and withstands PCR inhibitors without extensive purification.

Supplier: VWR Chemicals

Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of Taq DNA Polymerase to a 25 µl PCR reaction.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

Supplier: VWR Chemicals

Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

Supplier: VWR Chemicals

Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.

Supplier: VWR Chemicals

This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

Supplier: VWR Chemicals

La solution d'extraction rapide d'ADN permet une extraction prompte et efficace d'ADN prêt pour la PCR à partir de tissus de mammifères. L'ADN est prêt en huit minutes. Le protocole à un seul réactif est divisé en deux étapes simples de chauffage, qui peuvent être directement suivies d'une analyse PCR, comme le dépistage et le génotypage.

Supplier: VWR Chemicals

FAST HiFi DNA Polymerase 2 U/µl est une ADN polymérase de relecture présentant les caractéristiques suivantes : haute fidélité >60X Taq ADN Polymérase, la capacité d'amplifier des cibles d'ADN problématiques, telles que celles avec un contenu GC faible à élevé et la capacité d'effectuer l'amplification sur des cibles d'ADN longues. Elle est recommandée pour les applications, qui exigent une fidélité extrêmement élevée comme le clonage/le sous-clonage, les applications NGS et la mutagenèse..

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl est une ADN polymérase de relecture présentant les caractéristiques suivantes : haute fidélité >60X Taq ADN Polymérase, la capacité d'amplifier des cibles d'ADN problématiques, telles que celles avec un contenu GC faible à élevé et la capacité d'effectuer l'amplification sur des cibles d'ADN longues. Elle est recommandée pour les applications, qui exigent une fidélité extrêmement élevée comme le clonage/le sous-clonage, les applications NGS et la mutagenèse.

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® est un cocktail de réaction concentré 2X, prêt à l'emploi pour l'amplification PCR de matrices d'ADN qui évite les inhibiteurs souvent présents dans les échantillons bruts extraits de prélèvements environnementaux et de tissus végétaux ou animaux. Il s'agit d'un réactif de PCR polyvalent et robuste qui offre une amplification spécifique et efficace pour le génotypage de variables de séquences notamment la discrimination allélique de polymorphismes de nucléotide simple (SNP) à l'aide de nombreuses compositions chimiques de sonde fluorogénique, notamment les sondes d'hydrolyse TaqMan®.

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

Supplier: Quantabio

Superior speed and inhibitor tolerance.

Supplier: Apollo Scientific

Recombinant Taq plus DNA polymerase produced in E.coli 5 U/µl.

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

Supplier: VWR Chemicals

Taq DNA polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kDa.