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You searched for: End-point PCR Enzymes and Kits

Master mixes, multiplex, Phusion® U

Master mixes, multiplex, Phusion® U

Supplier: FINNZYMES REAGENTS

Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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Master mixes, green multiplex, Phusion® U

Master mixes, green multiplex, Phusion® U

Supplier: FINNZYMES REAGENTS

Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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Taq DNA polymerase (recombinant)

Taq DNA polymerase (recombinant)

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.

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Taq DNA polymerase (native, without BSA)

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli.

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Taq DNA polymerase (native, with BSA)

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli. Native Taq DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

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Taq DNA polymerase, MP Biomedicals

Supplier: MP Biomedicals

Taq DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

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Billes PCR, illustra™ PuReTaq Ready-To-Go™

Billes PCR, illustra™ PuReTaq Ready-To-Go™

Supplier: Cytiva

PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

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Pfu DNA polymerase

Pfu DNA polymerase

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

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ADN polymérase

Supplier: Merck Millipore (Novagen)

L'ADN polymérase KOD HiFi est une enzyme de correction unique, isolé de l'hyperthermophile Thermococcus kodakaraensis KOD1, qui possède une processivité et une fidélité supérieures permettant une amplification PCR plus rapide et plus précise que les enzymes conventionnels.

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Cloned Pfu DNA polymerase alternative detergents

Supplier: AGILENT

Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

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AMPIGENE® Taq DNA polymerase

Supplier: ENZO LIFE SCIENCES

Optimised standard PCR for enhanced speed, yield, and specificity.

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AMPIGENE® HS Taq DNA Polymerase

Supplier: ENZO LIFE SCIENCES

Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.

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PicoMaxx high fidelity PCR system

Supplier: AGILENT

Detects low copy number target and withstands PCR inhibitors without extensive purification.

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Pfu DNA polymerase

Supplier: AGILENT

High-quality polymerases for difficult to amplify DNA targets.

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VWR®, Taq DNA polymerase, 500 U

VWR®, Taq DNA polymerase, 500 U

Supplier: VWR Chemicals

Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of Taq DNA Polymerase to a 25 µl PCR reaction.

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ADN polymérase TEMPase Hot Start

ADN polymérase TEMPase Hot Start

Supplier: VWR Chemicals

Les polymérases ADN TEMPase Hot Start VWR® sont très stables, avec une plus grande spécificité, une sensibilité plus élevée et de meilleurs rendements par rapport aux polymérases ADN standard. Grâce à ces caractéristiques, elles sont particulièrement adaptées à la détection des cibles peu abondantes. D'autres utilisations concernent le dépistage, l'amplification de séquences riches en GC, la PCR multiplexe et la PCR et la qPCR directe. L'ADN polymérase sans glycérol TEMPase Hot Start est également disponible pour l'automatisation et la lyophilisation.

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Tampons PCR

Tampons PCR

Supplier: VWR Chemicals

Un système optimal de tampon est essentiel pour réussir la PCR. Les kits de Taq ADN polymérase VWR® incluent généralement deux tampons différents, un tampon Key et un tampon Extra qui sont adaptés aux différents besoins de la PCR. Tous les tampons contiennent du Tris et 15 mM de MgCl₂ (Concentration finale de 1,5 mM MgCl₂). MgCl₂ en complément pour faciliter l'optimisation est incluse dans une fiole séparée.

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VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix

VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

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ADN polymérase Taq sans glycérol, VWR®

ADN polymérase Taq sans glycérol, VWR®

Supplier: VWR Chemicals

L'ADN polymérase Taq hautement concentrée, sans glycérol, 50 U/µl, est idéale pour l'utilisation industrielle et la fabrication de kits de diagnostic PCR.

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AccuStart™ II PCR ToughMix®

AccuStart™ II PCR ToughMix®

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

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MycoSensor qPCR assay kits

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

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AccuStart™ Genotyping ToughMix®

AccuStart™ Genotyping ToughMix®

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® est un cocktail de réaction concentré 2X, prêt à l'emploi pour l'amplification PCR de matrices d'ADN qui évite les inhibiteurs souvent présents dans les échantillons bruts extraits de prélèvements environnementaux et de tissus végétaux ou animaux. Il s'agit d'un réactif de PCR polyvalent et robuste qui offre une amplification spécifique et efficace pour le génotypage de variables de séquences notamment la discrimination allélique de polymorphismes de nucléotide simple (SNP) à l'aide de nombreuses compositions chimiques de sonde fluorogénique, notamment les sondes d'hydrolyse TaqMan®.

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Solution d'extraction rapide d'ADN, VWR®

Solution d'extraction rapide d'ADN, VWR®

Supplier: VWR Chemicals

La solution d'extraction rapide d'ADN permet une extraction prompte et efficace d'ADN prêt pour la PCR à partir de tissus de mammifères. L'ADN est prêt en huit minutes. Le protocole à un seul réactif est divisé en deux étapes simples de chauffage, qui peuvent être directement suivies d'une analyse PCR, comme le dépistage et le génotypage.

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VWR® Fast extract genotyping PCR kit

VWR® Fast extract genotyping PCR kit

Supplier: VWR Chemicals

This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

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FAST HiFi DNA Polymerase, Master mix (2X), VWR®

FAST HiFi DNA Polymerase, Master mix (2X), VWR®

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl est une ADN polymérase de relecture présentant les caractéristiques suivantes : haute fidélité >60X Taq ADN Polymérase, la capacité d'amplifier des cibles d'ADN problématiques, telles que celles avec un contenu GC faible à élevé et la capacité d'effectuer l'amplification sur des cibles d'ADN longues. Elle est recommandée pour les applications, qui exigent une fidélité extrêmement élevée comme le clonage/le sous-clonage, les applications NGS et la mutagenèse.

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FAST HiFi DNA Polymerase, 2 U/µl

FAST HiFi DNA Polymerase, 2 U/µl

Supplier: VWR Chemicals

FAST HiFi DNA Polymerase 2 U/µl est une ADN polymérase de relecture présentant les caractéristiques suivantes : haute fidélité >60X Taq ADN Polymérase, la capacité d'amplifier des cibles d'ADN problématiques, telles que celles avec un contenu GC faible à élevé et la capacité d'effectuer l'amplification sur des cibles d'ADN longues. Elle est recommandée pour les applications, qui exigent une fidélité extrêmement élevée comme le clonage/le sous-clonage, les applications NGS et la mutagenèse..

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VWR®, Red Taq DNA polymerase master mix

VWR®, Red Taq DNA polymerase master mix

Supplier: VWR Chemicals

VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

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Taq ADN polymérase

Taq ADN polymérase

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

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VWR®, Taq DNA polymerase glycerol free

VWR®, Taq DNA polymerase glycerol free

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

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VWR®, Taq Plus 2X master mixes

VWR®, Taq Plus 2X master mixes

Supplier: VWR Chemicals

VWR® Taq Plus is an optimised format of Taq DNA polymerase master mix and, therefore, is a good alternative to Taq DNA polymerase and Taq DNA polymerase master mix.

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