Vous avez recherché : End-point PCR Enzymes and Kits

Supplier: VWR Chemicals

Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of Taq DNA Polymerase to a 25 µl PCR reaction.

Supplier: VWR Chemicals

Les polymérases ADN TEMPase Hot Start VWR® sont très stables, avec une plus grande spécificité, une sensibilité plus élevée et de meilleurs rendements par rapport aux polymérases ADN standard. Grâce à ces caractéristiques, elles sont particulièrement adaptées à la détection des cibles peu abondantes. D'autres utilisations concernent le dépistage, l'amplification de séquences riches en GC, la PCR multiplexe et la PCR et la qPCR directe. L'ADN polymérase sans glycérol TEMPase Hot Start est également disponible pour l'automatisation et la lyophilisation.

Supplier: VWR Chemicals

Un système optimal de tampon est essentiel pour réussir la PCR. Les kits de Taq ADN polymérase VWR® incluent généralement deux tampons différents, un tampon Key et un tampon Extra qui sont adaptés aux différents besoins de la PCR. Tous les tampons contiennent du Tris et 15 mM de MgCl₂ (Concentration finale de 1,5 mM MgCl₂). MgCl₂ en complément pour faciliter l'optimisation est incluse dans une fiole séparée.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: VWR Chemicals

L'ADN polymérase Taq hautement concentrée, sans glycérol, 50 U/µl, est idéale pour l'utilisation industrielle et la fabrication de kits de diagnostic PCR.

Supplier: VWR Chemicals

VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

Supplier: VWR Peqlab

PCR products of up to 12 kb and up to 40 kb can be achieved with the high performance peqGOLD 'Mid Range' and 'Long Range' PCR Systems respectively, suitable for both genomic and episomal templates. Applicable in place of standard Taq DNA polymerase for PCR amplifications when longer fragments and larger amounts are desirable, including sequencing, mapping and λ-phage or cosmid studies.

Supplier: VWR Chemicals

VWR® Taq Plus is an optimised format of Taq DNA polymerase master mix and, therefore, is a good alternative to Taq DNA polymerase and Taq DNA polymerase master mix.

Supplier: VWR Chemicals

Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® est un cocktail de réaction concentré 2X, prêt à l'emploi pour l'amplification PCR de matrices d'ADN qui évite les inhibiteurs souvent présents dans les échantillons bruts extraits de prélèvements environnementaux et de tissus végétaux ou animaux. Il s'agit d'un réactif de PCR polyvalent et robuste qui offre une amplification spécifique et efficace pour le génotypage de variables de séquences notamment la discrimination allélique de polymorphismes de nucléotide simple (SNP) à l'aide de nombreuses compositions chimiques de sonde fluorogénique, notamment les sondes d'hydrolyse TaqMan®.

Supplier: VWR Chemicals

La solution d'extraction rapide d'ADN permet une extraction prompte et efficace d'ADN prêt pour la PCR à partir de tissus de mammifères. L'ADN est prêt en huit minutes. Le protocole à un seul réactif est divisé en deux étapes simples de chauffage, qui peuvent être directement suivies d'une analyse PCR, comme le dépistage et le génotypage.

Supplier: VWR Chemicals

This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl est une ADN polymérase de relecture présentant les caractéristiques suivantes : haute fidélité >60X Taq ADN Polymérase, la capacité d'amplifier des cibles d'ADN problématiques, telles que celles avec un contenu GC faible à élevé et la capacité d'effectuer l'amplification sur des cibles d'ADN longues. Elle est recommandée pour les applications, qui exigent une fidélité extrêmement élevée comme le clonage/le sous-clonage, les applications NGS et la mutagenèse.

Supplier: VWR Chemicals

FAST HiFi DNA Polymerase 2 U/µl est une ADN polymérase de relecture présentant les caractéristiques suivantes : haute fidélité >60X Taq ADN Polymérase, la capacité d'amplifier des cibles d'ADN problématiques, telles que celles avec un contenu GC faible à élevé et la capacité d'effectuer l'amplification sur des cibles d'ADN longues. Elle est recommandée pour les applications, qui exigent une fidélité extrêmement élevée comme le clonage/le sous-clonage, les applications NGS et la mutagenèse..

Supplier: VWR Chemicals

Taq DNA polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kDa.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

Supplier: VWR Chemicals

Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.

Supplier: ENZO LIFE SCIENCES

Optimised standard PCR for enhanced speed, yield, and specificity.

Supplier: ENZO LIFE SCIENCES

Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.

Supplier: Quantabio

AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: AGILENT

Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.

Supplier: AGILENT

Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Supplier: AGILENT

Detect as low as 300 fg of Porcine DNA in food and pharmaceutical samples, including gel caps.

Supplier: ENZO LIFE SCIENCES

Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity.

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.