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140 results for Suppléments et additifs pour culture cellulaire

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Suppléments et additifs pour culture cellulaire

The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.

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Émulsion de jaune d'œuf avec tellurite

Émulsion de jaune d'œuf avec tellurite

Supplier: VWR Chemicals

Egg yolk tellurite emulsion, VWR®

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Cocktails d'inhibiteur de protéase, usage général

Cocktails d'inhibiteur de protéase, usage général

Supplier: VWR Chemicals

Ce produit contient un mélange d'inhibiteurs de protéase : AEBSF, aprotinine, E-64, bestatine et leupeptine. Chaque flacon peut être reconstitué dans 1 ml d'eau déionisée pour former une solution 100X.

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VWR®, Trypsin-EDTA solutions

VWR®, Trypsin-EDTA solutions

Supplier: VWR Chemicals

VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

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Glutamine

Glutamine

Supplier: Biowest

L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

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Protease inhibitor cocktail, Plant ProteaseArrest™

Protease inhibitor cocktail, Plant ProteaseArrest™

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

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Protease and phosphatase inhibitor, Protease-PhosphataseArrest™

Supplier: G-Biosciences

Protease-PhosphataseArrest™ provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

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Phosphatase inhibitor cocktails, PhosphataseArrest™

Supplier: G-Biosciences

The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

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Natural product library, Screen-Well®

Supplier: ENZO LIFE SCIENCES

Natural products are an unsurpassed source of chemical diversity and an ideal starting point for any screening program for pharmacologically active small molecules. Historically, natural products have been the most successful source of new drugs. The Screen-Well® natural product library offers over 500 isolated natural compounds of known structure and includes many compounds from ethnopharmacology. Compounds are provided in solution in DMSO. Individual compounds or subsets can be resupplied in gram quantities.

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TM-235 defined serum-free growth medium

TM-235 defined serum-free growth medium

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

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Cell dissociation reagents, HyrTryp™

Cell dissociation reagents, HyrTryp™

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

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L-Glutamine, HyClone™

Supplier: HyClone products (Cytiva)

HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.

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Protease inhibitor set

Protease inhibitor set

Supplier: G-Biosciences

Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.

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Protease inhibitor cocktail, FOCUS™ ProteaseArrest™

Protease inhibitor cocktail, FOCUS™ ProteaseArrest™

Supplier: G-Biosciences

A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

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M9 Minimal Salts (Maniatis Formulation), Powder, MP Biomedicals

M9 Minimal Salts (Maniatis Formulation), Powder, MP Biomedicals

Supplier: MP Biomedicals

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E.coli and plasmid amplification.

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Extrait de levure

Extrait de levure

Supplier: VWR Chemicals

La fraction soluble dans l'eau de levure autolysée contenant une source de vitamine B complexe.

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VWR® Protease Inhibitor Cocktail, Plant

VWR® Protease Inhibitor Cocktail, Plant

Supplier: VWR Chemicals

Contains a mix of protease inhibitors: AEBSF, E-64, Bestatin, Pepstatin, Leupeptin and 1, 10-Phenanthroline. Each vial can be reconstituted in 1 ml dimethyl sulfoxide to form a 100X solution.

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Matrice Corning® Matrigel®, sans rouge de phénol

Matrice Corning® Matrigel®, sans rouge de phénol

Supplier: Corning

La matrice Corning® Matrigel®, sans rouge de phénol, est recommandée pour les dosages nécessitant une détection colorimétrique (par exemple en fluorescence). La matrice de membrane basale Corning® Matrigel® est efficace pour la fixation et la différenciation des cellules épithéliales dépendantes du support à la fois normales et transformées, et d'autres types de cellules. Ces dernières incluent les neurones, les cellules de Sertoli, les cellules du cristallin de poussin, les cellules endothéliales vasculaires et les hépatocytes. La matrice Corning® Matrigel® influence l'expression génétique des hépatocytes chez le rat adulte, ainsi que la culture tridimensionnelle de cellules épithéliales mammaires chez la souris et chez l'humain. Elle facilite la régénération nerveuse périphérique in vivo, peut être utilisée pour des études du métabolisme et de toxicologie, et sert de base pour plusieurs types de tests d'invasion des cellules tumorales. La matrice Corning® Matrigel® fournit le substrat nécessaire pour l'étude de l'angiogenèse in vitro et in vivo. La matrice Corning® Matrigel® soutient également la propagation in vivo de tumeurs humaines chez des souris immunosuprimées.

   Sustainable Options Available
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MEM non essential amino acids 100X

MEM non essential amino acids 100X

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

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Versene

Versene

Supplier: Biowest

Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.

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Protease inhibitor cocktail

Supplier: ENZO LIFE SCIENCES

Protease inhibitor cocktail

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TCH defined serum-free growth medium

TCH defined serum-free growth medium

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement used primarily for human cells and the production of cell secreted proteins. TCH™ is also effective in culturing other mammalian cells without the use of animal proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, recombinant insulin), which are heat treated.

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Serum-free growth medium, defined, HemaPro

Serum-free growth medium, defined, HemaPro

Supplier: PROTIDE PHARMACEUTICALS

Serum supplement for the culturing of human progenitor cells. Effective in studying cancer stem cells, iPSC, and mesenchymal stem cells.

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Protease inhibitor cocktails, ProteCEASE™

Supplier: G-Biosciences

ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

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Yeast extract, molecular biology grade

Supplier: PanReac AppliChem

Water-soluble part of autolysed yeast. Rich of ammonia and vitamins, especially of water-soluble vitamin B-complexes; suitable for addition to media.

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Cryopreservation solutions, DMSO-free, pZerve™

Cryopreservation solutions, DMSO-free, pZerve™

Supplier: PROTIDE PHARMACEUTICALS

A unique cryopreservation solution that does not contain DMSO, foetal bovine serum, or animal protein. pZerve™ is ready to use and does not require any dilution or further processing.

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HyClone Cell Boost™ 7b supplement

HyClone Cell Boost™ 7b supplement

Supplier: HyClone products (Cytiva)

Supplements cell culture with concentrated amino acids. Manufactured to meet cGMP manufacturing standards and QC specifications.

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Protease inhibitor cocktail, Recom ProteaseArrest™

Protease inhibitor cocktail, Recom ProteaseArrest™

Supplier: G-Biosciences

Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

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Ionotropic glutamatergic ligand library, Screen-Well®

Supplier: ENZO LIFE SCIENCES

The Screen-Well® ionotropic glutamatergic ligands plate contains 60 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The ionotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.

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Autophagy library, Screen-Well®

Supplier: ENZO LIFE SCIENCES

Structurally and mechanistically different compounds for studying the roles of pro- and anti-autophagic molecules in cells and in vitro applications.

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MEM vitamins 100X concentrate

MEM vitamins 100X concentrate

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

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