You searched for: Suppléments et additifs pour culture cellulaire

Supplier: ENZO LIFE SCIENCES

Accelerates drug optimisation with a unique collection of compounds of known safety and bioavailability for diverse targets.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® purinergic and adenosine ligands plate contains 72 CNS receptor ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The purinergic and adenosine ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.

Supplier: THERMO FISHER DIAGNOSTICS

Egg yolk emulsion

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Yeast/ Fungal ProteaseArrest™ inhibits yeast and fungal serine, cysteine and metalloproteases.

Supplier: Thermo Fisher Scientific

Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Supplier: G-Biosciences

TCM-ProteaseArrest™ is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic (acid) proteases and aminopeptidases.

Supplier: ENZO LIFE SCIENCES

The Screen-Well™ Kinase Inhibitor Library contains 80 known kinase inhibitors of well-defined activity. Inhibitors are supplied dissolved in DMSO at 10 mM and aliquoted into deep-well plates at either 100 or 500 ml per well. The library is an ideal tool for chemical genomics, assay development and other pharmacological applications. Includes inhibitors of these important kinases: Insulin/IGF Receptors, PI 3-Kinase, CaM Kinase II, JAK, PKA, CDK, JNK, PKC, CKI II, MAPK, RAF, EGFR, MEK, SAPK, GSK, MLCK, Src-family, IKK, PDGFR, VEGFR and many more.

Supplier: ENZO LIFE SCIENCES

The cholinergic ligands plate contains 72 compounds ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The cholinergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® hematopoietic toxicity library is a focused collection of 115 compounds with defined and diverse hematopoietic toxicity, including myelosuppression, neutropenia, leukopenia, anaemia, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.

Supplier: ENZO LIFE SCIENCES

The Screen-Well™ Protease Inhibitor Library includes 53 known protease inhibitors on a single 96-well plate. Inhibitors are supplied dissolved in DMSO at 10 mM and aliquoted into deep-well plates at either 100 or 500 microliters per well. The library includes inhibitors to a broad range of important proteases and is a convenient and cost-effective way to purchase a panel of protease inhibitors for chemical genomics, assay development, and other applications. It includes inhibitors of: Calpains, TPPII, Neutrophil Elastase, Cathepsins, DPPIV, Caspases, Granzyme B, gamma-Secretase, Furin, MMPs, ACE, Kallikrein, Proteasome, Aminopeptidase B, and many more.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® opioid and sigma ligands plate contains 74 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The opioid and sigma ligands plate is 1 plate of the larger Neurotransmitter Library which contains over 650 ligands.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® ion channel ligand library contains 71 ion channel blockers and openers for use in characterising and identifying ion channels in individual cells or tissue.

Supplier: ENZO LIFE SCIENCES

The GABAergic igands library contains 56 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The GABAergics ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.

Supplier: ENZO LIFE SCIENCES

Hydroxytyrosol acetate is an antioxidant comparable to resveratrol. It is converted to hydroxytyrosol through hydrolysis by esterases.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® metabotropic glutamatergic ligands plate contains 49 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The metabotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.

Supplier: Biowest

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Supplier: HyClone products (Cytiva)

SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.

Supplier: HyClone products (Cytiva)

Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: THERMO FISHER DIAGNOSTICS

Egg yolk tellurite emulsion

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: PROVITRO

The supplement kit designed for cultivating human smooth muscle cells (HSMC) provides an optimised formulation for promoting growth and maintaining the health of these cells. Upon adding the components of the supplement kit to a 500 ml basal medium, a specialised environment is created to support the culture of HSMCs. The formulation is specifically tailored for the initial seeding of 4000 cells per square centimeter, aiming to achieve confluence at approximately 90%. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. All components are subjected to rigorous biological assays. Each batch is specifically examined for the proliferating characteristics of human smooth muscle cells (HSMC). The cells cultured in the smooth muscle cell growth medium undergo thorough assessments, including examination of their morphology, adherence rate, colony-forming efficiency, and population doubling time.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: PROVITRO

The supplement kit for endothelial cell proliferation medium is suitable for culturing Provitro human endothelial cells after adding the supplement kit components to 500 ml basal medium The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. The supplement kit for endothelial cell priliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time.

Supplier: ENZO LIFE SCIENCES

The Screen-Well™ Phosphatase Inhibitor Library contains 33 known phosphatase inhibitors of well-defined activity. Inhibitors are supplied dissolved in DMSO at 10 mM and aliquoted into deep-well plates at either 100 µl or 500 µl per well. The library is ideal for chemical genomics, assay development, and as a reference set for secondary screening. Includes inhibitors of these important phosphatases: Calcineurin(PP2B), JSP-1, PRL-1, CD45, PP1, PRL-3, CDC25, PP2A, PTEN, and many more.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® Wnt pathway library v 2.0 is a focused collection of 71 compounds with defined and diverse Wnt pathway activity, including activators and inhibitors of Wnts, Dishevelled, GSK-3β and other Wnt pathway kinases, TCF/β-catenin, DKK, LRP, Axin, Porcupine, and more. Compounds are dissolved in DMSO at 10 mM or 1 mM and aliquoted into deep-well plates at 100 or 500 µl per well. A variety of structurally and mechanistically different compound classes are included. A useful tool for studying the roles of pro- and anti-Wnt pathway molecules in cells as well as for use in in vitro applications.

Supplier: ENZO LIFE SCIENCES

The adrenergic ligand library contains 84 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The adrenergic ligand library is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® neurotransmitter library contains 681 CNS receptor ligands in a 96-well format. Ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, validating new assays, and for routine pharmacological applications.

Supplier: Charles River Laboratories Cell Solutions

HypoThermosol® biopreservation media is a novel, engineered, optimised hypothermic storage and shipping media product. Serum-free, protein-free HypoThermosol® is designed to provide maximum storage and shipping stability for biologics at 2 to 8 °C.

Supplier: Thermo Fisher Scientific

Phosphorylation and dephosphorylation is a molecular on/off switch that regulates a number of key biological pathways within the cell, including signal transduction, cell division, and apoptosis. The Halt Phosphatase Inhibitor Cocktail preserves phosphorylation of proteins in cell lysates and tissue extracts.