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Antibody purification buffers are necessary components in the antibody purification process, which involves selective enrichment of specific isolation of antibodies from serums, ascites fluids, or cell cultures supernatant of a hybridoma cell line. These buffers ensure high-yield, nondenaturing antibody purification, and are often provided in specialized formulations for purification of specific antibodies. Antibody purification buffers are pre-filtered and delivered ready to use, and products are available for each antibody purification method.
Citrate buffer, pH 6.0, 10×, antigen retriever, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Citrate buffer, antigen retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization.
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Tris-HCl buffer, pH 10, 10×, antigen retriever, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.
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Diluent buffer, 10X concentrate, for dab membrane substrate
Supplier: Rockland Immunochemicals
Concentrate diluent buffer for DAB membrane substrate, 10X
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Antibody diluent (HAMA blocker) for ELISA
Supplier: Abcam
Antibody diluent (HAMA blocker) for ELISA is a proprietary reagent for immunoassays involving a sandwich antibody complex that are susceptible to heterophilic antibody interference.
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Binding and elution buffers for antibody purification, Pierce™
Supplier: Thermo Fisher Scientific
These buffers ensure high-yield, non denaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.
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His·Bind® buffer kit, Novagen®
Supplier: Merck Millipore (Novagen)
The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.
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Buffer kit for antibody purification on protein A column
Supplier: Thermo Fisher Scientific
Buffer kit for antibody purification
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Ab Buffer Kit
Supplier: Cytiva
Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.