Tampons d'extraction
Les tampons d’extraction sont prêts à l’emploi et efficaces pour l’isolement de l’ARN total et d’autres protéines, améliorant le rendement protéique et la complexité des échantillons à partir de cellules et de tissus. Des réactifs d’extraction optimisés favorisent l’extraction des protéines dans des tampons natifs ou dénaturants compatibles avec les techniques d’analyse en aval. Une solution tampon appropriée ajoutée à un mélange de protéines pendant le processus d’extraction peut améliorer la stabilité des molécules protéiques lorsqu’elles sont soumises à diverses forces visant à les isoler pour étude.
I-PER™ Insect Cell Protein Extraction Reagents
Supplier: Thermo Fisher Scientific
The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
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IP lysis buffer, Pierce™
Supplier: Thermo Fisher Scientific
The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.
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Luciferase cell lysis buffer (2X), Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
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Protein solubilisation buffer
Supplier: G-Biosciences
FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.
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Protein extraction kit, Yeast PE LB™
Supplier: G-Biosciences
Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.
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T-PER™ Tissue Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable
Supplier: Thermo Fisher Scientific
This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
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ReadiUse™ Mammalian Cell Lysis Buffer *5X*
Supplier: AAT Bioquest
All our ReadiUse™ reagents require minimal hands-on time.
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DNA/RNA clean-up buffers
Supplier: OMEGA BIO-TEK
Tampon CP, Omega Bio-Tek
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Tampons et réactifs pour la recherche
Supplier: G-Biosciences
Ces tampons et réactifs sont exempts de DNase et RNase; ils s'adressent à la recherche de haut niveau.
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Y-PER™ Yeast Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.
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Subcellular protein fractionation kit for tissues
Supplier: Thermo Fisher Scientific
This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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Protein extraction kit, Insect PE LB™
Supplier: G-Biosciences
Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)
Supplier: AAT Bioquest
Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
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ReadiUse™ Yeast Cell Lysis Buffer, 5X
Supplier: AAT Bioquest
ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.



