Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Succinate, 0,2 M buffer solution, pH 5,0

Supplier: Thermo Fisher Scientific

Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific

Expand 2 Items

NP-40 lysis buffer (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer (2X), Thermo Fisher Scientific

Expand 1 Items

Triton® X-100 lysis buffer with glycerol (2X), pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific

Expand 1 Items

ACK Lysing Buffer (1x), TheraPEAK™

Supplier: LONZA

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.

Expand 1 Items

NP-40 lysis buffer with glycerol (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific

Expand 1 Items

NP-40 lysis buffer

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, Thermo Fisher Scientific

Expand 2 Items

Triton® X-100 lysis buffer, pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific

Expand 2 Items

RIPA Lysis and Extraction Buffer

Supplier: G-Biosciences

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.

Expand 2 Items

2-D Protein Extraction Buffer

Supplier: Cytiva

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.