Vous avez recherché : Tampons d'extraction

Supplier: G-Biosciences

Ces tampons et réactifs sont exempts de DNase et RNase; ils s'adressent à la recherche de haut niveau.

Supplier: VWR Chemicals

Tampon sans détergent pour l'isolement des protéines des cellules de culture tissulaire. La lyse cellulaire et l'isolement conséquent de la fraction cytoplasmique brute sont réalisés en moins de 30 minutes.

Supplier: OMEGA BIO-TEK

Tampon EB, Omega Bio-Tek

Supplier: OMEGA BIO-TEK

Tampon CP, Omega Bio-Tek

Supplier: Merck Millipore (Novagen)

BugBuster® 10X est une formulation concentrée des détergents exclusifs utilisés dans BugBuster® sans ajout de sels ou de composants tampon. Le réactif BugBuster® concentré offre une alternative flexible au réactif BugBuster® 1X standard prêt à l'emploi, permettant ainsi une dilution personnalisée et l'ajout de composants tampon. BugBuster® 10X présente tous les avantages du réactif BugBuster® standard en matière de biotraitement, mais il offre également la liberté de contrôler le pH, la concentration en réactif et les ajouts de tampon nécessaires pour une activité et une extraction maximales de vos protéines cibles.

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

Supplier: ENZO LIFE SCIENCES

Cell lysis buffer 4, Enzo Life Sciences

Supplier: AGILENT

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

Supplier: ENZO LIFE SCIENCES

Concentrated lysis buffer for preparation of cell and tissue extracts.

Supplier: ENZO LIFE SCIENCES

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

Supplier: ENZO LIFE SCIENCES

RIPA cell lysis buffer 2, Enzo Life Sciences

Supplier: OMEGA BIO-TEK

Le réactif stabilisateur E.Z.N.A.® RNALock est un réactif innovant qui lyse rapidement les cellules et les tissus pour désactiver les RNases à température ambiante. Il suffit d'immerger les échantillons, tels que les cultures cellulaires, les tissus animaux ou les globules blancs, dans le réactif stabilisateur RNALock. L'ARN cellulaire intact sera préservé même à température élevée. Les échantillons peuvent être conservés dans ce réactif pendant plus de 12 mois à -20 °C.

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

Supplier: ENZO LIFE SCIENCES

Caspase cell lysis buffer, Enzo Life Sciences

Supplier: PanReac AppliChem

Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

Supplier: Thermo Fisher Scientific

The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.

Supplier: Thermo Fisher Scientific

The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.

Supplier: OMEGA BIO-TEK

RBB Buffer, 4000 ml, Omega Bio-Tek

Supplier: Thermo Fisher Scientific

The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

Supplier: G-Biosciences

Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Supplier: Thermo Fisher Scientific

Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: G-Biosciences

Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Supplier: AAT BIOQUEST

Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.