Tampons d'extraction

Supplier: AAT Bioquest

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Supplier: G-Biosciences

Ces tampons et réactifs sont exempts de DNase et RNase; ils s'adressent à la recherche de haut niveau.

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Supplier: Thermo Fisher Scientific

RBC lysis buffer for mouse, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

For cell lysis and protein extraction.

Supplier: Thermo Fisher Scientific

Denaturing lysis buffer, Thermo Fisher Scientific

Supplier: Rockland Immunochemicals

Cell lysis buffer is used to lyse cells under non-denaturing conditions.

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

Le réactif d'extraction de protéines BugBuster® est formulé de façon à rompre délicatement la paroi cellulaire de l'E. coli, afin de libérer la protéine soluble. Il offre une alternative simple, rapide et économique aux méthodes mécaniques (French Press ou sonication) d'extraction de protéines cibles à des fins de purification ou d'autres applications. La formulation utilise un mélange de détergents capable de perforer la paroi cellulaire sans dénaturer la protéine soluble.

Supplier: Thermo Fisher Scientific

RBC lysis buffer for human, Thermo Fisher Scientific

Supplier: G-Biosciences

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.

Supplier: Merck Millipore (Novagen)

BugBuster® 10X est une formulation concentrée des détergents exclusifs utilisés dans BugBuster® sans ajout de sels ou de composants tampon. Le réactif BugBuster® concentré offre une alternative flexible au réactif BugBuster® 1X standard prêt à l'emploi, permettant ainsi une dilution personnalisée et l'ajout de composants tampon. BugBuster® 10X présente tous les avantages du réactif BugBuster® standard en matière de biotraitement, mais il offre également la liberté de contrôler le pH, la concentration en réactif et les ajouts de tampon nécessaires pour une activité et une extraction maximales de vos protéines cibles.