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Vecteurs, plasmides et banques

Vecteurs, plasmides et banques

En clonage moléculaire, les vecteurs sont des molécules d’ADN utilisées pour introduire artificiellement du matériel génétique étranger dans une autre cellule. Les quatre principaux types de vecteurs sont les plasmides, les vecteurs viraux, les cosmides et les chromosomes artificiels, les plasmides étant les plus utilisés. Les vecteurs peuvent être employés pour le clonage, certains étant spécifiquement conçus à cet effet, tandis que d’autres sont destinés à la transcription et à l’expression de protéines. Les vecteurs de transcription plus simples servent à amplifier le matériel génétique inséré.

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Clone ORF humain étiqueté, SGOL2 (SGO2)

Supplier: OriGene

SGOL2 (Myc-DDK-tagged)-Human shugoshin-like 2 (S. pombe) (SGOL2), variante de transcription 1. Ce clone a été conçu pour exprimer l'ORF complet avec un marquage d'expression. L'expression varie en fonction de la nature du gène.

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Human Tagged ORF Clone, SLC52A3

Supplier: OriGene

Lenti ORF particles, SLC52A3 (mGFP-tagged)-Human chromosome 20 open reading frame 54 (C20orf54), 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Tagged ORF Clone, GPR172B (SLC52A1)

Supplier: OriGene

Lenti ORF particles, SLC52A1 (mGFP-tagged) - Human G protein-coupled receptor 172B (GPR172B), transcript variant 1, 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Tagged ORF Clone Lentiviral Particle, PD-L1 (CD274)

Supplier: OriGene

Lenti ORF particles, CD274 (Myc-DDK tagged) - Human CD274 molecule (CD274), 200 µl, >10⁷ TU/ml.

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Human Tagged ORF Clone, GPCR MRGX2 (MRGPRX2)

Supplier: OriGene

MRGPRX2 (tGFP-tagged) - Human MAS-related GPR, member X2 (MRGPRX2). This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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AAV helper-free system

Supplier: AGILENT

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

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Human Tagged ORF Clone, CFTR (NM_000492)

Supplier: OriGene

This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Tagged ORF Clone, P Glycoprotein (ABCB1)

Supplier: OriGene

This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. ABCB1 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1).

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Human Tagged ORF Clone, GPR172A (SLC52A2)

Supplier: OriGene

Lenti ORF particles, SLC52A2 (mGFP-tagged) - Human G protein-coupled receptor 172A (GPR172A), 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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InterPlay Adenoviral TAP Systems

Supplier: AGILENT

The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

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Mouse Untagged Clone Plasmid, Lrrc15 (NM_028973)

Supplier: OriGene

Lrrc15 (untagged) - Mouse leucine rich repeat containing 15 (Lrrc15).

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Mammalian TAP system, interPlay

Supplier: AGILENT

The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

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Lambda DNA

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Lambda DNA is a temperate E. coli bacteriophage.

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