Milieux de culture cellulaire

Supplier: VWR Chemicals

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.

Supplier: VWR Chemicals

L'albumine de sérum bovin (BSA) VWR® est utilisée dans de nombreuses applications comme agent stabilisant. L'albumine sert à solubiliser les lipides et est également utilisée comme agent de blocage dans les western blots et les tests ELISA. Elle est couramment utilisée dans les protocoles de culture cellulaire, principalement pour l'apport de protéines. Il peut également être ajouté aux milieux de transport viral pour stabilisation.

Supplier: VWR Chemicals

Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from Drosophila spp and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.

Supplier: VWR Chemicals

Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.

Supplier: VWR Chemicals

Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.

Supplier: VWR Chemicals

VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.

Supplier: VWR Chemicals

Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.

Supplier: VWR Chemicals

RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.

Supplier: VWR Chemicals

L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.

Supplier: VWR Chemicals

Le milieu Ham's F12 a été développé pour la culture d'hépatocytes primaires de rat et de cellules épithéliales de prostate de rat. Utilisé également dans un test de toxicité clonale sur cellules CHO. Il est fréquemment utilisé avec des suppléments pour les cultures sans sérum afin de favoriser la croissance de cellules d'origine rongeur, ou comme milieu de clonage pour la culture de cellules de myélome et de cellules hybrides.

Supplier: VWR Chemicals

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: Biowest

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: Apollo Scientific

Contains macro/micronutrients, sucrose, agar andorganics as described by Malmgren, 1996 Plant tissue culture testedFull specification given in appendix.

Supplier: HyClone products (Cytiva)

HyClone Classical Liquid Media: Iscove's Modified Dulbeccos Medium (IMDM) is manufactured using ISO 9001:2000-certified processes.

Supplier: HyClone products (Cytiva)

HyClone™ SFMTransfx-293 is a serum-free, animal derived component-free medium designed to support the growth of HEK 293 cultures and promote transfection using lipofection or similar methods. Manufactured according to cGMP guidelines.

Supplier: HyClone products (Cytiva)

HyClone™ SFM4MAb media was developed through the Metabolic Pathway Design approach to increase process yields for manufacturing of human and humanised recombinant antibodies for therapeutic use.