168 Results for: "vwr chemical buffers"

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

Supplier: VWR Chemicals

Disodium oxalate ≥99.8%, AnalaR NORMAPUR® analytical reagent

Supplier: VWR Chemicals

An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

Supplier: VWR Chemicals

Formaldehyde 4% in aqueous solution, VOLUSOL®, buffered pH 7.2 -7.6

Supplier: VWR Chemicals

DPBS is commonly used salt solution in cell enumeration as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS is also used in FACS (Fluorescence-Activated Cell Sorting) process to wash and re-suspend cells during the dissociation process, as well as for many analytical methods or biochemical treatments.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH. Universally applicable buffers for biochemistry must be water soluble, not interfere with biological processes or biological.

Supplier: VWR Chemicals

This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

Supplier: VWR Chemicals

These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.

Supplier: VWR Chemicals

Formalin pink ready to use fixative for tissue samples. Pots can be used for storage and transport.
Contains traces of pink dye to help see section.

Supplier: VWR Chemicals

Phenol red : ammonium sulphate (0.002 : 0.005) % in aqueous solution Reag. Ph. Eur. 1063603, buffered pH 4.7

Supplier: VWR Chemicals

Zinc acetate 0.25 mol/l Reag. Ph. Eur. 1102301, buffered with ammonium acetate pH 6.4

Supplier: VWR Chemicals

The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.

Supplier: VWR Chemicals

An ideal replacement for water-saturated phenol in many protocols. Offers the high purity and performance of water saturated phenol with greatly enhanced stability and safety.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: VWR Chemicals

Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.

Supplier: VWR Chemicals

Phenol red : ammonium sulphate (0.002 : 0.01) % in aqueous solution Reag. Ph. Eur. 1063604, buffered pH 4.7

Supplier: VWR Chemicals

peqGOLD pre-stained Protein Marker VI is a mixture of 10 coloured proteins. These proteins are recombinantly produced in E.coli, highly purified and supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS polyacrylamide gels.

Supplier: VWR Chemicals

Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

Supplier: VWR Chemicals

PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.

Supplier: VWR Chemicals

Potassium dihydrogen phosphate 1/15 mol/l (1/15 M), AVS TITRINORM buffer stock solution

Supplier: VWR Chemicals

Packaged at pH 6.6, this mixture is provided with a supplemental alkaline buffer that allows the researcher to easily increase the pH to 7.9 for DNA purification. When mixed with Chloroform:Isoamyl Alcohol (24:1), it makes an optimised reagent for DNA purification. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.

Supplier: VWR Chemicals

Every MediaBag T (tube-bag) is filled with pre-weighed granulated media. Connect the 0,2 μm filter unit aseptically to the tube by using the connector device. Add water under aseptic conditions into the bag via the filter system. Protect the tube end with the red cap.

Supplier: VWR Chemicals

EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.

Supplier: VWR Chemicals

peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.

Supplier: VWR Chemicals

Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.

Supplier: VWR Chemicals

MES (β-(N-Morpholino)ethanesulphonic acid), anhydrous ≥99%, high purity suitable for buffer solutions